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List of All Publications:


  1. Vilchèze C, Kim J, Jacobs WR Jr. (2018). “Vitamin C potentiates the killing of Mycobacterium tuberculosis by the first-line tuberculosis drugs isoniazid and rifampicin in mice.” Antimicrob Agents Chemother. PMID: 29298757

  2. Burn, C., Ramsey, N., Garforth, S.J., Almo, S., Jacobs, W.R., Jr. and Herold, B.C. (2017). "An HSV-2 single-cycle candidate vaccine deleted in glycoprotein D, DeltagD-2, protects male mice from lethal skin challenge with clinical isolates of HSV-1 and HSV-2". J Infect Dis. PMID: 29216362.

  3. Hanauer, D.I., Graham, M.J., Betancur, L., Bobrownicki, A., Cresawn, S.G., Garlena, R.A., Jacobs-Sera, D., Kaufmann, N., Pope, W.H., Russell, D.A., Jacobs, W.R., Jr., Sivanathan, V., Asai, D.J. and Hatfull, G.F. (2017). "An inclusive Research Education Community (iREC): Impact of the SEA-PHAGES program on research outcomes and student learning". Proc Natl Acad Sci U S A. PMID: 2920871

  4. Retamal-Diaz, A., Weiss, K.A., Tognarelli, E.I., Freire, M., Bueno, S.M., Herold, B.C., Jacobs, W.R., Jr. and Gonzalez, P.A. (2017). "US6 Gene Deletion in Herpes Simplex Virus Type 2 Enhances Dendritic Cell Function and T Cell Activation". Front Immunol. (8)1523. PMID: 5686121

  5. Stratton, T.P., Perryman, A.L., Vilcheze, C., Russo, R., Li, S.G., Patel, J.S., Singleton, E., Ekins, S., Connell, N., Jacobs, W.R., Jr. and Freundlich, J.S. (2017). "Addressing the Metabolic Stability of Antituberculars through Machine Learning". ACS Med Chem Lett. 8(10):1099-1104. PMID: 29057058

  6. Kunnath-Velayudhan, S., Goldberg, M.F., Saini, N.K., Johndrow, C.T., Ng, T.W., Johnson, A.J., Xu, J., Chan, J., Jacobs, W.R., Jr. and Porcelli, S.A. (2017). "Transcriptome Analysis of Mycobacteria-Specific CD4+ T Cells Identified by Activation-Induced Expression of CD154". J Immunol. 199(7):2596-2606. PMID: 28821584

  7. Liu, R., Lyu, X., Batt, S.M., Hsu, M.H., Harbut, M.B., Vilcheze, C., Cheng, B., Ajayi, K., Yang, B., Yang, Y., Guo, H., Lin, C., Gan, F., Wang, C., Franzblau, S.G., Jacobs, W.R., Jr., Besra, G.S., Johnson, E.F., Petrassi, M., Chatterjee, A.K., Futterer, K. and Wang, F. (2017). "Determinants of the Inhibition of DprE1 and CYP2C9 by Antitubercular Thiophenes". Angew Chem Int Ed Engl. PMID: 28815830

  8. Glass, L.N., Swapna, G., Chavadi, S.S., Tufariello, J.M., Mi, K., Drumm, J.E., Lam, T.T., Zhu, G., Zhan, C., Vilcheze, C., Arcos, J., Chen, Y., Bi, L., Mehta, S., Porcelli, S.A., Almo, S.C., Yeh, S.R., Jacobs, W.R., Jr., Torrelles, J.B. and Chan, J. (2017). "Mycobacterium tuberculosis universal stress protein Rv2623 interacts with the putative ATP binding cassette (ABC) transporter Rv1747 to regulate mycobacterial growth". PLoS Pathog. 13(7):e1006515. PMID: 28753640

  9. Yang, Y., Thomas, J., Li, Y., Vilcheze, C., Derbyshire, K.M., Jacobs, W.R., Jr. and Ojha, A.K. (2017). "Defining a temporal order of genetic requirements for development of mycobacterial biofilms". Mol Microbiol. PMID: 28628249

  10. Saito, K., Warrier, T., Somersan-Karakaya, S., Kaminski, L., Mi, J., Jiang, X., Park, S., Shigyo, K., Gold, B., Roberts, J., Weber, E., Jacobs, W.R., Jr. and Nathan, C.F. (2017). "Rifamycin action on RNA polymerase in antibiotic-tolerant Mycobacterium tuberculosis results in differentially detectable populations". Proc Natl Acad Sci U S A. 114(24):E4832-E4840. PMID:  28559332 

  11. Qaqish, A., Huang, D., Chen, C.Y., Zhang, Z., Wang, R., Li, S., Yang, E., Lu, Y., Larsen, M.H., Jacobs, W.R., Jr., Qian, L., Frencher, J., Shen, L. and Chen, Z.W. (2017). "Adoptive Transfer of Phosphoantigen-Specific gammadelta T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates". J Immunol. 198(12):4753-4763. PMID: 28526681

  12. Vilcheze, C., Hartman, T., Weinrick, B., Jain, P., Weisbrod, T.R., Leung, L.W., Freundlich, J.S. and Jacobs, W.R., Jr. (2017). "Enhanced respiration prevents drug tolerance and drug resistance in Mycobacterium tuberculosis". Proc Natl Acad Sci U S A. 114(17):4495-4500. PMID:  28396391

  13. Ryan, A., Polycarpou, E., Lack, N.A., Evangelopoulos, D., Sieg, C., Halman, A., Bhakta, S., Eleftheriadou, O., McHugh, T.D., Keany, S., Lowe, E.D., Ballet, R., Abuhammad, A., Jacobs, W.R., Jr., Ciulli, A. and Sim, E. (2017). "Investigation of the mycobacterial enzyme HsaD as a potential novel target for anti-tubercular agents using a fragment-based drug design approach". Br J Pharmacol. 174(14):2209-2224. PMID:  28380256

  14. Prados-Rosales, R., Carreno, L., Cheng, T., Blanc, C., Weinrick, B., Malek, A., Lowary, T.L., Baena, A., Joe, M., Bai, Y., Kalscheuer, R., Batista-Gonzalez, A., Saavedra, N.A., Sampedro, L., Tomas, J., Anguita, J., Hung, S.C., Tripathi, A., Xu, J., Glatman-Freedman, A., Jacobs, W.R., Jr., Chan, J., Porcelli, S.A., Achkar, J.M. and Casadevall, A. (2017). "Enhanced control of Mycobacterium tuberculosis extrapulmonary dissemination in mice by an arabinomannan-protein conjugate vaccine". PLoS Pathog. 13(3):e1006250. PMID: 5360349

  15. Kerantzas, C.A. and Jacobs, W.R., Jr. (2017). "Origins of Combination Therapy for Tuberculosis: Lessons for Future Antimicrobial Development and Application". MBio. 8(2): PMID: 28292983

  16. Johnson, A.J., Kennedy, S.C., Lindestam Arlehamn, C.S., Goldberg, M.F., Saini, N.K., Xu, J., Paul, S., Hegde, S.S., Blanchard, J.S., Chan, J., Jacobs, W.R., Jr., Sette, A. and Porcelli, S.A. (2017). "Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4+ T Cells". Infect Immun. 85(4): PMID: 27655885

  17. Jensen, K., Dela Pena-Ponce, M.G., Piatak, M., Jr., Shoemaker, R., Oswald, K., Jacobs, W.R., Jr., Fennelly, G., Lucero, C., Mollan, K.R., Hudgens, M.G., Amedee, A., Kozlowski, P.A., Estes, J.D., Lifson, J.D., Van Rompay, K.K., Larsen, M. and De Paris, K. (2017). "Balancing Trained Immunity with Persistent Immune Activation and the Risk of Simian Immunodeficiency Virus Infection in Infant Macaques Vaccinated with Attenuated Mycobacterium tuberculosis or Mycobacterium bovis BCG Vaccine". Clin Vaccine Immunol. 24(1):PMID:5216431

  18. Korte J, Alber M, Trujillo CM, Syson K, Koliwer-Brandl H, Deenen R, Köhrer K, DeJesus MA, Hartman T, Jacobs WR Jr, Bornemann S, Ioerger TR, Ehrt S, Kalscheuer R. (2016). Trehalose-6-Phosphate-Mediated Toxicity Determines Essentiality of OtsB2 in Mycobacterium tuberculosis In Vitro and in Mice. PLoS Pathogens (12):e1006043. PMID: 27936238

  19. Portal-Celhay C, Tufariello JM, Srivastava S, Zahra A, Klevorn T, Grace PS, Mehra A, Park HS, Ernst JD, Jacobs WR Jr, Philips JA. (2016). Mycobacterium tuberculosis EsxH inhibits ESCRT-dependent CD4+ T-cell activation. Nature Microbiology 2:16232. PMID: 27918526

  20. Maggioli MF, Palmer MV, Thacker TC, Vordermeier HM, McGill JL, Whelan AO, Larsen MH, Jacobs WR Jr, Waters WR. (2016) Increased TNF-α/IFN-γ/IL-2 and Decreased TNF-α/IFN-γ Production by Central Memory T Cells Are Associated with Protective Responses against Bovine Tuberculosis Following BCG Vaccination.  Frontiers in Immunology PMID: 27799930

  21. Jain P, Weinrick BC, Kalivoda EJ, Yang H, Munsamy V, Vilcheze C, Weisbrod TR, Larsen MH, O'Donnell MR, Pym A, Jacobs WR Jr. (2016). Dual-Reporter Mycobacteriophages (Φ2DRMs) Reveal Preexisting Mycobacterium tuberculosis Persistent Cells in Human Sputum. MBio. (5). pii: e01023-16. PMID: 27795387

  22. Mayer O, Jain P, Weisbrod TR, Biro D, Ho L, Jacobs-Sera D, Hatfull GF, Jacobs WR Jr. (2016)  Fluorescent Reporter DS6A Mycobacteriophages Reveal Unique Variations in Infectibility and Phage Production in Mycobacteria. J. Bacteriol. (23):3220-3232.  PMID: 27672191

  23. Foreman TW, Mehra S, LoBato DN, Malek A, Alvarez X, Golden NA, Bucşan AN, Didier PJ, Doyle-Meyers LA, Russell-Lodrigue KE, Roy CJ, Blanchard J, Kuroda MJ, Lackner AA, Chan J, Khader SA, Jacobs WR Jr, Kaushal D. (2016). CD4+ T-cell-independent mechanisms suppress reactivation of latent tuberculosis in a macaque model of HIV coinfection. Proc Natl Acad Sci (38):E5636-44. PMID: 27601645

  24. Vergnolle O, Xu H, Tufariello JM, Favrot L, Malek AA, Jacobs WR Jr, Blanchard JS. (2016). Post-translational Acetylation of MbtA Modulates Mycobacterial Siderophore Biosynthesis. J Biol Chem. (42):22315-22326. PMID: 27566542

  25. Saini NK, Baena A, Ng TW, Venkataswamy MM, Kennedy SC, Kunnath-Velayudhan S, Carreño LJ, Xu J, Chan J, Larsen MH, Jacobs WR Jr, Porcelli SA. (2016). Suppression of autophagy and antigen presentation by Mycobacterium tuberculosis PE_PGRS47. Nat Microbiol. (9):16133. PMID: 27562263

  26.  Petro CD, Weinrick B, Khajoueinejad N, Burn C, Sellers R, Jacobs WR Jr, Herold BC. (2016). HSV-2 ΔgD elicits FcγR-effector antibodies that protect against clinical isolates. JCI Insight (12). pii: e88529. PMID: 27536733

  27. Koliwer-Brandl, H., Syson, K., van de Weerd, R., Chandra, G., Appelmelk, B., Alber, M., Ioerger, T.R., Jacobs, W.R., Jr., Geurtsen, J., Bornemann, S. and Kalscheuer, R. (2016). "Metabolic Network for the Biosynthesis of Intra- and Extracellular alpha-Glucans Required for Virulence of Mycobacterium tuberculosis". PLoS Pathog. 12(8):e1005768. PMID:4981310

  28. Halloum I, Carrère-Kremer S, Blaise M, Viljoen A, Bernut A, Le Moigne V, Vilchèze C, Guérardel Y, Lutfalla G, Herrmann JL, Jacobs WR Jr, Kremer L. (2016). Deletion of a dehydratase important for intracellular growth and cording renders rough Mycobacterium abscessus avirulent. Proc Natl Acad Sci U S A. 113(29):E4228-37. PMID: 27385830

  29. Yu, X., Gu, Y., Jiang, G., Ma, Y., Zhao, L., Sun, Z., Jain, P., O'Donnell, M., Larsen, M., Jacobs, W.R., Jr. and Huang, H. (2016). "Evaluation of a High-Intensity Green Fluorescent Protein Fluorophage Method for Drug- Resistance Diagnosis in Tuberculosis for Isoniazid, Rifampin, and Streptomycin". Front Microbiol. 7(922). PMID:4911409

  30. Jensen K, Nabi R, Van Rompay KK, Robichaux S, Lifson JD, Piatak M Jr, Jacobs WR Jr, Fennelly G, Canfield D, Mollan KR, Hudgens MG, Larsen MH, Amedee AM, Kozlowski PA, De Paris K. (2016). Vaccine-Elicited Mucosal and Systemic Antibody Responses Are Associated with Reduced Simian Immunodeficiency Viremia in Infant Rhesus Macaques. J Virol. 90(16):7285-302. PMID: 27252535

  31. Olsen A, Chen Y, Ji Q, Zhu G, De Silva AD, Vilchèze C, Weisbrod T, Li W, Xu J, Larsen M, Zhang J, Porcelli SA, Jacobs WR Jr, Chan J. (2016). Targeting Mycobacterium tuberculosis Tumor Necrosis Factor Alpha-Downregulating Genes for the Development of Antituberculous Vaccines. MBio. 7(3). pii: e01023-15. PMID: 27247233.

  32. Prados-Rosales R, Carreño LJ, Weinrick B, Batista-Gonzalez A, Glatman-Freedman A, Xu J, Chan J, Jacobs WR Jr, Porcelli SA, Casadevall A. (2016). The Type of Growth Medium Affects the Presence of a Mycobacterial Capsule and Is Associated with Differences in Protective Efficacy of BCG Vaccination against Mycobacterium tuberculosis. J Infect Dis. (3):426-37. PMID: 2723441
    Noy T, Vergnolle O, Hartman TE, Rhee KY, Jacobs WR Jr, Berney M, Blanchard JS. (2016). Central Role of Pyruvate Kinase in Carbon Co-catabolism of Mycobacterium tuberculosis. J Biol Chem. 291(13):7060-9. PMCID: PMC4807288

  33. Waters WR, Maggioli MF, Palmer MV, Thacker TC, McGill JL, Vordermeier HM, Berney-Meyer L, Jacobs WR Jr, Larsen MH. (2015). "Interleukin-17A as a Biomarker for Bovine Tuberculosis". ASM Journals. 16;23(2):168-80. PMCID: PMC4744917                                                                      

  34. Tufariello JM, Chapman JR, Kerantzas CA, Wong KW, Vilchèze C, Jones CM, Cole LE, Tinaztepe E, Thompson V, Fenyö D, Niederweis M, Ueberheide B, Philips JA, Jacobs WR Jr. (2015). Separable Roles for Mycobacterium tuberculosis ESX-3 Effectors in Iron Acquisition and Virulence. PNAS. 113(3):E348-57. PMCID: PMC4725510

  35. Wong KW, Jacobs WR Jr. (2015). Postprimary tuberculosis and macrophage necrosis: is there any big conNECtion? MBio. 7(1)e01589-15. PMCID: PMC4724999

  36. Tufariello JM., Kerantzas CA., Vilchèze C., Calder RB., Nordberg EK., Fischer JA., Hartman TE., Yang E., Driscoll T., Cole LE., Sebra R., Maqbool SB., Wattam AR., Jacobs WR Jr. (2015). The Complete Genome Sequence of the Emerging Pathogen Mycobacterium haemophilum Explains Its Unique Culture Requirements. MBio. 6(6)e01313-15. PMID: 26578674

  37. Hart BE, Asrican R, Lim SY, Sixsmith JD, Lukose R, Souther SJ, Rayasam SD, Saelens JW, Chen CJ, Seay SA, Berney-Meyer L, Magtanong L, Vermeul K, Pajanirassa P, Jimenez AE, Ng TW, Tobin DM, Porcelli SA, Larsen MH, Schmitz JE, Haynes BF, Jacobs WR Jr, Lee S, Frothingham R. (2015) Stable Expression of Lentiviral Antigens by Quality-Controlled Recombinant BCG Vectors. CVI. 22(7):726-41. PMID: 25924766.PMCID: PMC4478521

  38. Vilcheze, C., L. W. Leung, R. Bittman, and W. R. Jacobs, Jr. (2015). Synthesis and biological activity of alkynoic acids derivatives against mycobacteria. Chem Phys Lipids. PMCID: PMC4718898.

  39. Berney, M., L. Berney-Meyer, K. W. Wong, B. Chen, M. Chen, J. Kim, J. Wang, D. Harris, J. Parkhill, J. Chan, F. Wang, and W. R. Jacobs, Jr. (2015). Essential roles of methionine and S-adenosylmethionine in the autarkic lifestyle of Mycobacterium tuberculosis. Proc Natl Acad Sci 112 (32):10008-13. PMCID: PMC4538671.

  40. Packiam, M., B. Weinrick, W. R. Jacobs, Jr., and A. T. Maurelli. (2015). Structural characterization of muropeptides from Chlamydia trachomatis peptidoglycan by mass spectrometry resolves "chlamydial anomaly". Proc Natl Acad Sci PMCID: PMC PMC4577195
    O’Donnell, M., Pym, A., Jain, P., Munsamy, V., Wolf, A., Karim, F., Jacobs, W., Larsen, M. (2015) A novel reporter phage to detect tuberculosis and rifampicin resistance in an HIV endemic population. J Clin Microbiol. JCM.03530-14. [Epub ahead of print] . PMCID: PMC 4473227.

  41. Ojha, A. K., W. R. Jacobs, Jr., and G. F. Hatfull. (2015). Genetic dissection of mycobacterial biofilms. Methods Mol Biol 1285 215-26. PMCID PMC (In process).

  42. Li, S. G., C. Vilcheze, S. Chakraborty, X. Wang, H. Kim, M. Anisetti, S. Ekins, K. Y. Rhee, W. R. Jacobs, Jr., and J. S. Freundlich. (2015). Evolution of a thienopyrimidine antitubercular relying on medicinal chemistry and metabolomics insights. Tetrahedron Lett 56 (23):3246-3250. PMCID: PMC4527344.

  43. Zolla, V., I. T. Nizamutdinova, B. Scharf, C. C. Clement, D. Maejima, T. Akl, T. Nagai, P. Luciani, J. C. Leroux, C. Halin, S. Stukes, S. Tiwari, A. Casadevall, W. R. Jacobs, Jr., D. Entenberg, D. C. Zawieja, J. Condeelis, D. R. Fooksman, A. A. Gashev, and L. Santambrogio. (2015). Aging-related anatomical and biochemical changes in lymphatic collectors impair lymph transport, fluid homeostasis, and pathogen clearance. Aging Cell.

  44. Chandrabos, C., S. M'Homa Soudja, B. Weinrick, M. Gros, A. Frangaj, M. Rahmoun, W. R. Jacobs, Jr., and G. Lauvau. (2015). The p60 and NamA autolysins from Listeria monocytogenes contribute to host colonization and induction of protective memory. Cell Microbiol 17 (2):147-63. PMCID: PMC4457399.

  45. Harbut, M. B., C. Vilcheze, X. Luo, M. E. Hensler, H. Guo, B. Yang, A. K. Chatterjee, V. Nizet, W. R. Jacobs, Jr., P. G. Schultz, and F. Wang. (2015). Auranofin exerts broad-spectrum bactericidal activities by targeting thiol-redox homeostasis. Proc Natl Acad Sci U S A 112 (14):4453-8.  PMCID PMC4394260.

  46. Pope, W.H., Bowman, C.A., Russell, D.A., Jacobs-Sera, D., Asai, D.J., Cresawn, S.G., Jacobs, W.R., Jr., Hendrix, R.W., Lawrence, J.G., Hatfull, G.F. (2015) Whole genome comparison of a large collection of mycobacteriophages revails a continuum of phage genetic diversity.  eLife PMCID PMC 4408529.

  47. Petro, C., González, P.A., Cheshenko, N., Jandl, T., Khajjoueinejad, N., Bénard, A., Sengupta, M., Herold, B.C., Jacobs, W.R., Jr. (2015)  Herpes simplex type 2 virus deleted in glycoprotein D protects against vaginal, skin and neural disease. eLife.  PMCID PMC4352706.

  48. Hartman, T., Weinrick, B.,  Vilcheze, C., Berney, M., Tufariello, J.M., G. M. Cook., and W. R. Jacobs, Jr. (2014) Succinate dehydrogenase is the regulator of respiration in Mycobacterium tuberculosis. PloS Pathogens. 10(11):e1004510. PMCID PMC 4161257.

  49. Pope, W. H., D. Jacobs-Sera, D. A. Russell, D. H. Rubin, A. Kajee, Z. N. Msibi, M. H. Larsen, W. R. Jacobs, Jr., J. G. Lawrence, R. W. Hendrix, and G. F. Hatfull. (2014). Genomics and proteomics of mycobacteriophage patience, an accidental tourist in the Mycobacterium neighborhood. MBio 5 (6):e02145.

  50. Panas, M. W., J. D. Sixsmith, K. White, B. Korioth-Schmitz, S. T. Shields, B. T. Moy, S. Lee, J. E. Schmitz, W. R. Jacobs, Jr., S. A. Porcelli, B. F. Haynes, N. L. Letvin, and G. O. Gillard. (2014). Gene Deletions in Mycobacterium bovis BCG Stimulate Increased CD8+ T Cell Responses. Infect Immun 82 (12):5317-26. PMCID PMC4249275.

  51. Prados-Rosales, R., L. J. Carreno, A. Batista-Gonzalez, A. Baena, M. M. Venkataswamy, J. Xu, X. Yu, G. Wallstrom, D. M. Magee, J. LaBaer, J. M. Achkar, W. R. Jacobs, Jr., J. Chan, S. A. Porcelli, and A. Casadevall. (2014). Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis. MBio 5 (5):e01921-14. PMCID PMC:4196239.

  52. Venkataswamy, M. M., T. W. Ng, S. S. Kharkwal, L. J. Carreno, A. J. Johnson, S. Kunnath-Velayudhan, Z. Liu, R. Bittman, P. J. Jervis, L. R. Cox, G. S. Besra, X. Wen, W. Yuan, M. Tsuji, X. Li, D. D. Ho, J. Chan, S. Lee, R. Frothingham, B. F. Haynes, M. W. Panas, G. O. Gillard, J. D. Sixsmith, B. Korioth-Schmitz, J. E. Schmitz, M. H. Larsen, W. R. Jacobs, Jr., and S. A. Porcelli. (2014). Improving Mycobacterium bovis bacillus Calmette-Guerin as a vaccine delivery vector for viral antigens by incorporation of glycolipid activators of NKT cells. PLoS One 9 (9):e108383. PMCID PMC 4177913.

  53. Chandrabos, C., S. M'Homa Soudja, B. Weinrick, M. Gros, A. Frangaj, M. Rahmoun, W. R. Jacobs, Jr., and G. Lauvau. (2014). The p60 and NamA autolysins from Listeria monocytogenes contribute to host colonization and induction of protective memory. Cell Microbiol. PMCID PMC in process.

  54. Berney, M., C. Greening, R. Conrad, W. R. Jacobs, Jr., and G. M. Cook. (2014). An obligately aerobic soil bacterium activates fermentative hydrogen production to survive reductive stress during hypoxia. Proc Natl Acad Sci U S A111 (31):11479-84. PMCID PMC 4128101.

  55. Panas, M.W., Jain, P., Yang, H., Mitra, S., Biswas, D., Wattam, A.R., Letvin, N.L., Jacobs, W.R.,Jr.  (2014)   Noncanonical SMC protein in Mycobacterium smegmatis restricts maintenance of Mycobacterium fortuitum plasmidsProc Natl Acad Sci. 111 (37):13264-71. PMCID PMC 4169951.

  56. Cheshenko N, Trepanier JB, González PA, Eugenin EA, Jacobs WR Jr, Herold BC. (2014) “Herpes Simplex Virus Type 2 Glycoprotein H Interacts with Integrin αvβ3 to Facilitate Viral Entry and Calcium Signaling in Human Genital Tract Epithelial Cells.” J Virol 88 (17):10026-38. PMCID PMC 4136333.

  57. Santha Kumar, T.R., Annoura, T., March, S., Chakravarty, S., Cobbold, SA., Matthews, K.A., Henrich, P.P., Li, T., Llinas, M., Janse, C.J., Khan, S.M., Sim, B.K.L., Bhatia, S.N., Hoffman, S.L., Fidock, D.A. (2014) The Fatty Acid Elongation Enzyme Elo1 is Non-Essential Throughout the Plasmodium Life Cycle. JB CHem (Submitted).

  58. Stec, J., C. Vilcheze, S. Lun, A. L. Perryman, X. Wang, J. S. Freundlich, W. Bishai, W. R. Jacobs, Jr., and A. P. Kozikowski. (2014). Biological Evaluation of Potent Triclosan-Derived Inhibitors of the Enoyl-Acyl Carrier Protein Reductase InhA in Drug-Sensitive and Drug-Resistant Strains of Mycobacterium tuberculosis. ChemMedChem. PMCID PMC 4213240.

  59. Sixsmith, J. D., M. W. Panas, S. Lee, G. O. Gillard, K. White, M. A. Lifton, H. Balachandran, L. Mach, J. P. Miller, C. Lavine, C. T. DeMarco, G. D. Tomaras, C. Gee, S. A. Porcelli, M. H. Larsen, R. Frothingham, J. E. Schmitz, W. R. Jacobs, Jr., B. F. Haynes, N. L. Letvin, and B. Korioth-Schmitz. (2014). Recombinant Bacillus Calmette-Guerin vectors prime for strong cellular responses to SIV Gag in rhesus macaques. Clin Vaccine Immunol. PMCID PMC4266348.

  60. Pecsi, I., K. Hards, N. Ekanayaka, M. Berney, T. Hartman, W. R. Jacobs, Jr., and G. M. Cook. (2014). Essentiality of Succinate Dehydrogenase in Mycobacterium smegmatis and Its Role in the Generation of the Membrane Potential Under Hypoxia. mBio 5. PMCID PMC 4145680.   

  61. Berney, M., Hartman, T., Jacobs, W.R., Jr., (2014) Mycobacterium tuberculosis cytochrome bd oxidase mutant is hypersensitive to Bedaquiline. mBio 5 (4):e01275-14. PMCID PMC4161257.

  62. Bartek, I.L., Woolhiser, L.K., Baughn, A.D., Basaraba, R.J., Jacobs, W.R., Jr, Lenaerts, A.J., Voskuil, M.I. (2014) Mycobacterium tuberculosis Lsr2 is a global transcriptional regulator required for adaptation to changing oxygen levels and virulence. mBio 5 (3):e01106-14. PMCID PMC4049101.

  63. Tufariello, J.M., Malek, A.A., Vilcheze, C., Cole,L.E., Ratner, H.K. Gonzalez, P.A., Jain, P., Hatfull, G.F., Larsen, M.H., Jacobs, W..R. (2014) Enhanced Specialezed Transduction Using Recombineering in Mycobacterium tuberculosismBio 5:(3) e01179-14 PMCID PMC4045075.

  64. Jain, P., Hsu, T., Arai, M., Biermann, K. E., Thaler, D. S., Nguyen, A., Gonzalez, P., Tufariello, J.M., Kriakov, J., Chen, B., Larsen, M. H., Jacobs Jr, W. R., Jr. (2014) Specialized Transduction Designed for Precise High-throughput Unmarked Deletions in Mycobacterium tuberculosis. mBio 5 (3):e01245-14. PMCID PMC4049104.

  65. Vilcheze, C., Molle, V., Carrere-Kremer, S., Leiba, J., Mourey, L., Shenai, S., Baronian, G., Tufariello, J., Hartman, T., Veyron-churlet, R., Trivelli, X., Tiwari, S., Weinrick, B., Alland, D., Guerardel, Y., Jacobs, W.R. Jr., Kremer, L., (2014) Phosphorylation Signaling by Ser/Thr Kinases Regulates Virulence and Acid-Fast Staining in Mycobacterium tuberculosis. PLoS Pathogen.  10 (5):e1004115.  PMCID PMC4014462.

  66. Cortesia, C., Vilcheze, C., Bernut, A., Contreras, W., Gomes, K., deWaard, J., Jacobs, W.R.,Jr. Kremer, L., Takiff, H. (2014) Acetic Acid or Vinegar is an Effective Tuberculicodal Disinfectant. mBio 5(2) e00013-14. PMCID PMC 3940030.

  67. Prados-Rosales,R., Weinrick, B., Pique, D., Jacobs, W. Jr., Casadevall, A., Rodrigues, G. (2014)  Role for Mycobacterium tuberculosis membrane vesicles in iron acquisition. J. Bacteriol. 196 (6):1250-6. PMCID PMC 3957709.

  68. Wong K.W., Jacobs W.R. Jr. (2013) Reply to Wallis.  J Infect Dis 209 (4):628-9. PMCID PMC 3903376.

  69. Hingley-Wilson, S. M., D. Connell, K. Pollock, T. Hsu, E. Tchilian, A. Sykes, L. Grass, L. Potiphar, S. Bremang, O. M. Kon, W. R. Jacobs, Jr., and A. Lalvani. (2014). ESX1-dependent fractalkine mediates chemotaxis and Mycobacterium tuberculosis infection in humans. Tuberculosis (Edinb). 94 (3):262-70. PMCID PMC4066952.

  70. Ramon-Garcia, S., C. Vilcheze, L. E. Lim, C. Ng, W. R. Jacobs, Jr., and C. J. Thompson. (2014). Measurements of the in vitro anti-mycobacterial activity of ivermectin are method-dependent--authors' response. J Antimicrob Chemother. 69 (6):1725-6. PMCID PMC (in process).

  71. Smith, K.L.J., Saini, D., Bardarov, S., Larsen, M., Frothingham,R., Gandhi,N.R., Jacobs, W.R.Jr., Sturm, W., Lee, S. (2014) Reduced virulence of an extensively drug-resistant outbreak strain of Mycobacterium tuberculosis in a murine model. PLoS One. April 9 (4)e94953. PMCID PMC3986381.

  72. Junqueira-Kipnis AP, de Oliveira FM, Trentini MM, Tiwari S, Chen B, Resende DP, Silva BD, Chen M, Tesfa L, Jacobs WR Jr, Kipnis A. (2013)  Prime-boost with Mycobacterium smegmatis recombinant vaccine improves protection in mice infected with Mycobacterium tuberculosis. PLoS One  Nov 8;8(11):e78639. doi: 10.1371/journal.pone.0078639. PMCID:PMC3826754.

  73. Chapman R, Stutz H, Jacobs W Jr, Shephard E, Williamson AL. (2013) Priming with recombinant auxotrophic BCG expressing HIV-1 Gag, RT and Gp120 and boosting with recombinant MVA induces a robust T cell response in mice. PLoS One.  Aug 20;8(8):e71601. doi: 10.1371/journal.pone.0071601. PMCID:PMC3748047.

  74. Kozakiewicz, L., Y. Chen, J. Xu, Y. Wang, K. Dunussi-Joannopoulos, Q. Ou, J. L. Flynn, S. A. Porcelli, W. R. Jacobs, Jr., and J. Chan. (2013). B Cells Regulate Neutrophilia during Mycobacterium tuberculosis Infection and BCG Vaccination by Modulating the Interleukin-17 Response. PLoS Pathog 9 (7):e1003472. PMCID PMC 3708864.

  75. Chen, C. Y., S. Yao, D. Huang, H. Wei, H. Sicard, G. Zeng, H. Jomaa, M. H. Larsen, W. R. Jacobs, Jr., R. Wang, N. Letvin, Y. Shen, L. Qiu, L. Shen, and Z. W. Chen. (2013). Phosphoantigen/IL2 Expansion and Differentiation of Vgamma2Vdelta2 T Cells Increase Resistance to Tuberculosis in Nonhuman Primates. PLoS Pathog 9 (8):e1003501.  PMCID PMC 3744401.

  76. Wang,F., Sambandan, D., Halder, R., Wang, J.,  Batt, S., Weinrick, B.C., Ahmad, I.,  Yang, P.,  Zhang, Y., Kim, J.,Hassani, M., Huszar, S., Trefzer, C., Ma, Z., Kaneko, T., Mdluli, K.E., Franzblau, S.c Chatterjee, A., Johnsson, K., Mikusova, K., Besra, G., Fütterer, K., Jacobs,W.R., Jr. Schultz, P.G. (2013) Identification of a Small Molecule with Activity against Drug-resistant and Persistent Tuberculosis.  PNAS U S A 110 (27):E2510-7. PMCID: PMC3703973.

  77. Wilson, R., P. Kumar, V. Parashar, C. Vilcheze, R. Veyron-Churlet, J. S. Freundlich, S. W. Barnes, J. R. Walker, M. J. Szymonifka, E. Marchiano, S. Shenai, R. Colangeli, W. R. Jacobs, Jr., M. B. Neiditch, L. Kremer, and D. Alland. (2013). Antituberculosis thiophenes define a requirement for Pks13 in mycolic acid biosynthesis. Nat Chem Biol.9(8) 499-506. PMCID PMC 3720791.

  78. Vilchèze, C., Hartman, T., Weinrick, B., and Jacobs, W.R. (2013). Mycobacterium tuberculosis is extraordinarily sensitive to killing by a vitamin C-induced Fenton reaction. Nat. Commun 4:1881. PMCID PMC  3698613.

  79. Sambandan, D., Dao, D.N., Weinrick, B.C., Vilcheze, C., Grucha, S.S., Ojha, A., Kremer, L., Besra, G.S., Hatfull, G.F., Jacobs, W.R. (2013) Keto-Mycolic acid-dependent pellicle formation confers tolerance to drug-sensitive Mycobacterium tuberculosis. mBio. 4(3):e00222-13 PMCID: PMC3663190.

  80. Ly, D., Kasmar, A.G., Cheng, T., DeJong, A., Huang, S., Roy, S., Bhatt, A., vanSummeren, R.R., Altman, J.D., Jacobs, W.R., Adams, E.J., Minnaard, A. J., Porcelli, S.A., Moody, D.B. (2013)  CD1c tetramers and sicovery of M. tuberculosis phosphomycoketides reveal cellular antigen processing for the human CD1c system. J Exp Med 210 (4):729-41. PMCID: PMC3620358.

  81. Miallau, L., P. Jain, M. A. Arbing, D. Cascio, T. Phan, C. J. Ahn, S. Chan, I. Chernishof, M. Maxson, J. Chiang, W. R. Jacobs, Jr., and D. S. Eisenberg. (2013). Comparative proteomics identifies the cell-associated lethality of M. tuberculosis RelBE-like toxin-antitoxin complexes. Structure 21 (4):627-37. PMCID: PMC3791866.

  82. Chege, G.K., Burgers, W.A., Stutz, H., Meyers, A., Chapman, R., Kiravu, A., Bunjun, R., Shephard, E.G., Jacobs, W.R., Rybicki, E., Williamson, A. (2013) Robust immunity to a auxotrophic BCG-VLP prime-boost HIV vaccine candidate in a non-human primate model.  J Virol 87 (9):5151-60. PMCID: PMC3624307.

  83. Anderson, J. W., D. Sarantakis, J. Terpinski, T. R. Santha Kumar, H. C. Tsai, M. Kuo, A. L. Ager, W. R. Jacobs, Jr., G. A. Schiehser, S. Ekins, J. C. Sacchettini, D. P. Jacobus, D. A. Fidock, and J. S. Freundlich. (2013). Novel diaryl ureas with efficacy in a mouse model of malaria. Bioorg Med Chem Lett 23:1022-5. PMCID PMC 3746744.

  84. Lim, L. E., C. Vilcheze, C. Ng, W. R. Jacobs, Jr., S. Ramon-Garcia, and C. J. Thompson. (2013). Anthelmintic Avermectins Kill Mycobacterium tuberculosis, Including Multidrug-Resistant Clinical Strains. Antimicrob Agents Chemother 57:1040-6. PMCID PMC 3553693.

  85. Cheshenko, N.,  Trepanier,J.B.,  Stefanidou, M., Buckley, N., Gonzalez, P., Jacobs,W.,  Herold, B.C.  (2013)  HSV Activates Akt to Trigger Calcium Release and Promote viral entry: Novel candidate target for treatment and suppression. FASEB J. 27(7):2584-99). PMCID: PMC3688744.

  86. Wong, K-W., Jacobs, W.R. Jr. (2013) Mycobacterium tuberculosis exploits human interferon-γ to stimulate macrophage extracellular trap formation and necrosis. J Infect Dis 208 (1):109-19 PMCID PMC3666134.

  87. Sayahi, H., K. M. Pugliese, O. Zimhony, W. R. Jacobs, Jr., A. Shekhtman, and J. T. Welch. (2012). Analogs of the Antituberculous Agent Pyrazinamide Are Competitive Inhibitors of NADPH Binding to M. tuberculosis Fatty Acid Synthase I. Chem Biodivers 9:2582-96. PMCID PMC.

  88. Ahmad, Z., S. Tyagi, A. Minkowsk, D. Almeida, E. L. Nuermberger, K. M. Peck, J. T. Welch, A. S. Baughn, W. R. Jacobs, Jr., and J. H. Grosset. (2012). Activity of 5-chloro-pyrazinamide in mice infected with Mycobacterium tuberculosis or Mycobacterium bovis. Indian J Med Res 136 (5):808-14. PMCID: PMC3573602.

  89. Vilcheze, C., Jacobs, W.R. Jr. (2012) The combination of sulfamethoxazole, trimethoprim, and isoniazid or rifampin is bactericidal and prevents the emergence of drug resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother . 56: (10) 5142-8.  PMCID PMC 3457372.

  90. Taylor, N., F. Bahunde, A. Thompson, J. S. Yu, W. R. Jacobs, N. L. Letvin, B. F. Haynes, and S. Lee. (2012). Enhanced priming of adaptive immunity by high secretor mutants of Mycobacterium smegmatis. Clin Vaccine Immunol PMCID PMC 3428392.

  91. Jensen, K., U. D. Ranganathan, K. K. Van Rompay, D. R. Canfield, I. Khan, R. Ravindran, P. A. Luciw, W. R. Jacobs, Jr., G. Fennelly, M. Larsen, and K. Abel. (2012). A recombinant attenuated Mycobacterium tuberculosis vaccine strain is safe in immunosuppressed SIV-infected infant macaques. Clin Vaccine Immunol PMCID PMC 3416096.

  92. Kolibab, K., S. C. Derrick, W. R. Jacobs, and S. L. Morris. (2012). Characterization of an intracellular ATP assay for evaluating the viability of live attenuated mycobacterial vaccine preparations. J Microbiol Methods 90:245-9. PMCID PMC.

  93. Yamada, H., A. Bhatt, R. Danev, N. Fujiwara, S. Maeda, S. Mitarai, K. Chikamatsu, A. Aono, K. Nitta, W. R. Jacobs, Jr., and K. Nagayama. (2012). Non-acid-fastness in Mycobacteriumtuberculosis DeltakasB mutant correlates with the cell envelope electron density. Tuberculosis (Edinb) PMCID PMC.

  94. Derrick, S. C., D. Dao, A. Yang, K. Kolibab, W. R. Jacobs, and S. L. Morris. (2012). Formulation of a mmaA4 Gene Deletion Mutant of Mycobacterium bovis BCG in Cationic Liposomes Significantly Enhances Protection against Tuberculosis. PLoS One 7:e32959. PMCID PMC 3307709.

  95. Goldberg, D.E., Siliciano,R.F., and Jacobs, W.R.Jr. (2012). Outwitting Evolution: Fighting Drug-Resistant TB, Malaria, and HIV. Cell 148:1271-83. PMCID PMC 3322542.

  96. McShane, H., W. R. Jacobs, P. E. Fine, S. G. Reed, D. N. McMurray, M. Behr, A. Williams, and I. M. Orme. (2012). BCG: myths, realities, and the need for alternative vaccine strategies. Tuberculosis (Edinb) 92 (3):283-8.

  97. Jain, P., Hartman, T.,  Eisenberg, N., O'Donnell, M.R., Kriakov, J.,  Govender, K.,  Makume, M., Thaler, D., Hatfull, G.F., Sturm, A.W.,  Larsen, M.H., Moodley, P., Jacobs,W.R. Jr. (2012) ф2GFP10: A high-intensity fluorophage enables detection and rapid drug susceptibility testing of Mycobacterium tuberculosis directly from sputum samples. J Clin Microbiol 50:1362-9. PMCID PMC 3318544.

  98. Venkataswamy, M. M., M. F. Goldberg, A. Baena, J. Chan, W. R. Jacobs, Jr., and S. A. Porcelli. (2012). In vitro culture medium influences the vaccine efficacy of Mycobacterium bovis BCG. Vaccine 30:1038-49. PMCID PMC 3269512.

  99. Chapman, R., Shepard, E., Stutz, H., Douglass, N., Sambandamurthy, V., Garcia, I., Ryffel, B., Jacobs, W., Williamson, A. (2012) Priming with a Recombinant Pathtothenate Auxotroph of Mycobacterium bovis BCG and Boosting with MVA Elicits HIV-1 Gag Specific CD8+ TCells.  PLoS One, 7:3 e32769. 

  100. Bourai, N., W. R. Jacobs, Jr., and S. Narayanan. (2012). Deletion and overexpression studies on DacB2, a putative low molecular mass penicillin binding protein from Mycobacterium tuberculosis H(37)Rv. Microb Pathog 52:109-16. PMCID PMC.

  101. Yu, J. S., J. Whitesides, S. H. Lee, N. Taylor, W. R. Jacobs, Jr., N. L. Letvin, and B. F. Haynes. (2011). Flow cytometry sorting of recombinant mycobacterial species yields bacterial clones with enhanced insert expression. Clin Vaccine Immunol 18 (1):43-9.PMCID PMC3019783.

  102. Hinchey, J., B. Y. Jeon, H. Alley, B. Chen, M. Goldberg, S. Derrick, S. Morris, W. R. Jacobs, Jr., S. A. Porcelli, and S. Lee. (2011). Lysine auxotrophy combined with deletion of the SecA2 gene results in a safe and highly immunogenic candidate live attenuated vaccine for tuberculosis. PLoS One 6 (1):e15857.PMCID PMC3018466

  103. Rondon, L., M. Piuri, W. R. Jacobs, Jr., J. de Waard, G. F. Hatfull, and H. Takiff. (2011). Evaluation of Fluoromycobacteriophages for detecting drug resistance in Mycobacterium tuberculosis. J Clin Microbiol.49:1838-42.  PMCID PMC 3122682.

  104. Prados-Rosales, R., A. Baena, L. R. Martinez, J. Luque-Garcia, R. Kalscheuer, U. Veeraraghavan, C. Camara, J. D. Nosanchuk, G. S. Besra, B. Chen, J. Jimenez, A. Glatman-Freedman, W. R. Jacobs, Jr., S. A. Porcelli, and A. Casadevall. (2011). Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice. J Clin Invest. PMCID PMC3069770.

  105. Xu, X., Vilcheze, C., Av-Gay, Y., Gomez-Velasco, A., and Jacobs, W.R. Jr., (2011) Precise Null Deletion Mutations of the Mycothiol Synthesis Genes Reveal Their Roll in Isoniazid and Ethionamide Resistance in Mycobacterium smegmatis. Antimicrob Agents Chemother. 55 (7):3133-9. PMCID PMC 3122461.

  106. Sweeney, K. A., D. N. Dao, M. F. Goldberg, T. Hsu, M. M. Venkataswamy, M. Henao-Tamayo, D. Ordway, R. S. Sellers, P. Jain, B. Chen, M. Chen, J. Kim, R. Lukose, J. Chan, I. M. Orme, S. A. Porcelli, and W. R. Jacobs, Jr. (2011). A recombinant Mycobacterium smegmatis induces potent bactericidal immunity against Mycobacterium tuberculosis. Nat Med 17:1261-8. PMCID PMC 3250071.

  107. Wong,K.W., and Jacobs,W.R. (2011) Critical role for NLRP3 in necrotic death triggered by M. tuberculosis. Cell Microbio. 13 (9):1371-84. PMCID PMC 3257557.

  108. Vilchèze, C., Baughn, A.D., Tufariello, J., Leung,L., Kuo, M., Basler, C., Alland, D., Sacchettini, J.C., Freunlich, J.S., and Jacobs, W.R.Jr. (2011)  Novel inhibitors of InhA efficiently kill Mycobacterium tuberculosis under aerobic and anaerobic conditions. Antimicrob Agents Chemother. 55 (8):3889-98. PMCID  PMC 3147652.

  109. Sayahi, H., O. Zimhony, W. R. Jacobs, Jr., A. Shekhtman, and J. T. Welch. (2011). Pyrazinamide, but not pyrazinoic acid, is a competitive inhibitor of NADPH binding to Mycobacterium tuberculosis fatty acid synthase I. Bioorg Med Chem Lett. 21:4804-7. PMCID PMC.

  110. Pruksakorn, P., M. Arai, L. Liu, P. Moodley, W. R. Jacobs, Jr., and M. Kobayashi. (2011). Action-Mechanism of Trichoderin A, an Anti-dormant Mycobacterial Aminolipopeptide from Marine Sponge-Derived Trichoderma sp. Biol Pharm Bull 34 (8):1287-90. PMCID PMC (in process).

  111. Pope, W. H., C. M. Ferreira, D. Jacobs-Sera, R. C. Benjamin, A. J. Davis, R. J. DeJong, S. C. R. Elgin, F. R. Guilfoile, M. H. Forsyth, A. D. Harris, S. E. Harvey, L. E. Hughes, P. M. Hynes, A. S. Jackson, M. D. Jalal, E. A. MacMurray, C. M. Manley, M. J. McDonough, J. L. Mosier, L. J. Osterbann, H. S. Rabinowitz, C. N. Rhyan, D. A. Russell, M. S. Saha, C. D. Shaffer, S. E. Simon, E. F. Sims, I. G. Tovar, E. G. Weisser, J. T. Wertz, K. A. Weston-Hafer, K. E. Williamson, B. Zhang, S. G. Cresawn, P. Jain, M. Piuri, W. R. Jacobs, Jr., R. W. Hendrix, and G. F. Hatfull. (2011). Cluster K Mycobacteriophages: Insights into the Evolutionary Origins of Mycobacteriophage TM4. PLoS One 6:e26750. PMCID PMC 3203893.

  112. Chen, B., Weisbrod, T.R., Hsu, T., Sambandamurthy, V., Viera-Cruz, D., Chibbaro, A., Ghidoni, D., Kile, T., Barkley, W.E., Vilcheze, C., Colon-Berezin, C., Thaler, D., Larsen, M.H., Sturm, A.W., Jacobs. W.R. Jr.(2011) Einstein Contained Aerosol Pulmonizer (ECAP): Inproved Biosafety for Multi-Drug Resistant (MDR) and Extensively Drug Resistant (XDR) Mycobacterium tuberculosis Aerosol Infection Studies. Applied Biosafety 16 (3):134-138. PMCID PMC.

  113. Parra, M., S. C. Derrick, A. Yang, J. Tian, K. Kolibab, M. Oakley, L. P. Perera, W. R. Jacobs, S. Kumar, and S. L. Morris. (2011). Malaria Infections Do Not Compromise Vaccine-Induced Immunity against Tuberculosis in Mice. PLoS One 6:e28164. PMCID PMC. 3242757.

  114. Jain, P., D. S. Thaler, M. Maiga, G. S. Timmins, W. R. Bishai, G. F. Hatfull, M. H. Larsen, and W. R. Jacobs. (2011). Reporter phage and breath tests: emerging phenotypic assays for diagnosing active tuberculosis, antibiotic resistance, and treatment efficacy. J Infect Dis 204 Suppl 4:S1142-50. PMCID PMC 3247800.

  115. Vilcheze, C., Y. Av-Gay, S. W. Barnes, M. H. Larsen, J. R. Walker, R. J. Glynne, and W. R. Jacobs, Jr. (2011). Coresistance to isoniazid and ethionamide maps to mycothiol biosynthetic genes in Mycobacterium bovis. Antimicrob Agents Chemother 55:4422-3. PMCID PMC. 3165297.

  116. Vilcheze, C., B. Weinrick, K. W. Wong, B. Chen, and W. R. Jacobs, Jr. (2010). NAD+ auxotrophy is bacteriocidal for the tubercle bacilli. Mol Microbiol 76:365-77. PMCID PMC2945688.

  117. Kalscheuer, R., Syson, K., Veeraraghavan, U., Weinrick, B., Biermann, K.E., Liu, Z., Sacchettini, J.C., Besra, G., Bornemann, S., Jacobs, W.R., Jr. (2010) Self-poisoning of Mycobacterium tuberculosis by inhibition of GlgE reveals a new class of drug target. Nature Chem Bio. 6(5):376-84. PMCID PMC3256575.

  118. Larsen, M.H., Jacobs, W.R., Porcelli, S.A, Kim, J., Ranganathan, U.D., Fennelly G. J. (2010) Balancing safety and immunogenicity in live-attenuated mycobacterial vaccines for use in humans at risk for HIV: response to misleading comments in Ranganathan et al. “recombinant pro-apoptotic Mycobacterium tuberculosis generates CD8+ T Cell responses against human immunodeficiency virus type 1 Env and M. tuberculosis in neonatal mice:. Vaccine. 28(21): 3633-3634. PMCID  PMC (in process).

  119. Kinhikar,A.G., Verma, I., Chandra, D., Singh, K.K., Weldingh, K., Andersen, P., Hsu, T., Jacobs, W.R., Jr., Laal, S. (2010) Potential role for ESAT6 in dissemination of M. tuberculosis via human lung epithelial cells. Mol. Microbiol. (75)1:92-106. PMCID PMC 2846543.

  120. Ioerger, T. R., Y. Feng, K. Ganesula, X. Chen, K. M. Dobos, S. Fortune, W. R. Jacobs, Jr., V. Mizrahi, T. Parish, E. Rubin, C. Sassetti, and J. C. Sacchettini. (2010). Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories. J Bacteriol 192:3645-53. PMCID PMC2897344.

  121. Pruksakorn, P., M. Arai, N. Kotoku, C. Vilcheze, A. D. Baughn, P. Moodley, W. R. Jacobs, Jr., and M. Kobayashi. (2010). Trichoderins, novel aminolipopeptides from a marine sponge-derived Trichoderma sp., are active against dormant mycobacteria. Bioorg Med Chem Lett 20:3658-63. PMCID PMC.

  122. Wang, F., P. Jain, G. Gulten, Z. Liu, Y. Feng, K. Ganesula, A. S. Motiwala, T. R. Ioerger, D. Alland, C. Vilcheze, W. R. Jacobs, Jr., and J. C. Sacchettini. (2010). Mycobacterium tuberculosis Dihydrofolate Reductase is Not a Target Relevant to the Anti-tubercular Activity of Isoniazid. Antimicrob Agents Chemother PMCID PMC2935018.

  123. Lazar-Molnar, E., B. Chen, K. A. Sweeney, E. J. Wang, W. Liu, J. Lin, S. A. Porcelli, S. C. Almo, S. G. Nathenson, and W. R. Jacobs, Jr. (2010). Programmed death-1 (PD-1)-deficient mice are extraordinarily sensitive to tuberculosis. Proc Natl Acad Sci U S A.   107:13402-7. PMCID: PMC2922129.

  124. Baughn, A. D., J. Deng, C. Vilcheze, A. Riestra, J. T. Welch, W. R. Jacobs, Jr., and O. Zimhony. (2010). Mutually exclusive genotypes for pyrazinamide and 5-chloropyrazinamide resistance reveal a potential resistance proofing strategy. Antimicrob Agents Chemother PMCID PMC2981270.

  125. Koser, C. U., D. K. Summers, J. A. Archer, and W. R. Jacobs, Jr. (2010). Role of the Dihydrofolate Reductase DfrA (Rv2763c) in Trimethoprim-Sulfamethoxazole (Co-Trimoxazole) Resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother 54:4951-2. PMCID PMC.

  126. Kalscheuer, R., B. Weinrick, U. Veeraraghavan, G. S. Besra, and W. R. Jacobs, Jr. (2010). Trehalose-recycling ABC transporter LpqY-SugA-SugB-SugC is essential for virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A.PMCID PMC3003129.

  127. Molle, V., G. Gulten, C. Vilcheze, R. Veyron-Churlet, I. Zanella-Cleon, J. C. Sacchettini, W. R. Jacobs, Jr., and L. Kremer. (2010). Phosphorylation of InhA inhibits mycolic acid biosynthesis and growth of Mycobacterium tuberculosis. Mol Microbiol 78 (6):1591-605

  128. Kalscheuer, R., and W. R. Jacobs, Jr. (2010). The significance of GlgE as a new target for tuberculosis. Drug News Perspect 23 (10):619-24.

  129. Lee, W.L., Gold, B., Darby, C., Brot, N., Jiang, X, de Carvalho, L.P., Wellner, D., John, G., Jacobs, W.R., Jr., Nathan, C. (2009) Mycobacterium tuberculosis Expresses Methionine Sulfoxide Reductases A and B that Protect from Killing by Nitrite and Hypochlorite. Mol Microbiol  71:583-593. PMCID: PMC3697012.

  130. Lim, J., Derrick, S.C., Kolibab, K., Yang, A.L., Porcelli, S., Jacobs, W.R., Morris, S.L. (2009) Early Pulmonary Cytokine and Chemokine Responses in Mice Immunized with Three Different Vaccines Against Mycobacterium tuberculosis Determined by PCR Array. Clin Vaccine Immunol 16:122-126. PMCID: PMC2620659.

  131. Endsley, J.J., Waters, W.R., Palmer, M.V., Nonnecke, B.J., Thacker, T.C., Jacobs, W.R., Jr., Larsen, M.H., Hogg, A., Shell, E., McAlauy, M., Scherer, C.F., Coffey, T., Howard, C.J., Villareal-Ramos, B., Estes, D.M. (2009) The Calf Model of Immunity for Development of a Vaccine Against Tuberculosis. Vet Immunol Immunopathol  128(1-3):199-204. PMCID: PMC in process.

  132. Yam, K.C., D’Angelo, I., Kalscheuer, R., Zhu, H., Wang, J.X., Snieckusk, V., Ly, L.H., Converse, P. J., Jacobs, W.R., Jr., Strynadka, N., Eltis, L.D. (2009) Studies of a ring-cleaving dioxygenase illuminate the role of cholesterol metabolism in the pathogenesis of Mycobacterium tuberculosis. PLoS Pathog. 5 (3):e1000344 [Epub]. PMCID: PMC2652662.

  133. Cayabyab, M.J., Korioth-Schmitz B., Sun, Y., Carville, A., Balachandran, H., Miura, A., Carlson, K.R., Buzby, A.P., Haynes, B.F., Jacobs, W.R., Letvin, N.L. (2009) Recombinant Mycobacterium bovis BCG prime-recombinant adenovirus boost vaccination in rhesus monkeys elicits robust polyfunctional simian immunodeficiency virus-specific T-cell responses. J Virol. 83 (11):5505-5513 [Epub]. PMCID: PMC2681969.

  134. Waters WR, Palmer MV, Nonnecke BJ, Thacker TC, Scherer CFC, Estes DM, Hewinson RG, Vordermeier HM, Barnes SW, Federe GC, Walker JR, Glynne RJ, Hsu T, Weinrick B, Biermann K, Larsen MH, Jacobs WR, Jr.  (2009) Efficacy and immunogenicity of Mycobacterium bovis DRD1 against aerosol M. bovis infection in neonatal calves.  Vaccine 27:1201-1209. PMCID: PMC2750035.

  135. Freundlich, J.S., Wang, F., Vilcheze, C., Gulten, G., Langley, R., Schiehser, G.A., Jacobus, D.P., Jacobs, W.R., Jr., Sacchettini, J. (2009) Tricolosan Derivatives:  Towards Potent Inhibitors of Drug-Sensitive and Drug Resistant M. tuberculosis. Chem Med Chem  4:241-248.PMCID: PMC3541007.

  136. Parra, M., Yang, A.L., Lim, J., Kolibab, K., Kerrick, S., Cadieux, N., Perera, L.P., Jacobs, W.R., Brennan, M., Morris, S.L. (2009) The development of a Murine Mycobacterial Growth Inhibition assay for Evaluating Vaccines Against Mycobacterium tuberculosis. Clin Vaccine Immunol.  PMCID: PMC2708400.

  137. Chen, C.Y., Huang, D., Wang, R.C., Shen, L., Zeng, G., Yao, S., Shen, Y., Halliday, L., Fortman, J., McAllister, M., Estep, J., Hunt, R., Vasconcelos, D., Du, G., Porcelli, S.A., Larsen, M.H., Jacobs, W. J., Jr., Haynes, B. F., Letvin, N.L., Chen Z.W. (2009) A critical role ofr CD8 T cells in a nonhuman primate model of tuberculosis. PLoS Pathog. 5 (4):e1000392 [Epub]. PMCID: PMC2663842.

  138. Cirillo,. S.L., Subbian, S., Chen, B., Weisbrod, T.R.,  Cirillo, J.D. (2009) Protection of Mycobacterium tuberculosis from reactive oxygen species conferred by the mel2 locus impacts persistence and dissemination. Infect Immunol 77: (6) 2557-2567. PMCID: PMC2687327.

  139. Gopalaswamy, R., Narayanan, S., Chen, B., Jacobs, W.R., Av-Gay, Y. (2009) The serine/threonine protein kinase Pknl controls the growth of Mycobacterium tuberculosis upon infection. FEMS Microbiol Lett 295 (1): 23-29. PMCID: PMC in process.

  140. Piuri, M., Jacobs, W.R. Jr., Hatfull, G. F. (2009) Fluoromycobactericphages for repid, specific, and sensitive antibiotic susceptiblility testing of Mycobacterium tuberculosis. PLoS ONE 4 (3) e-4870. PMCID: PMC2654538.

  141. Capinos Scherer, C.F., Endsley, J.J., deAguiar, J.B., Jacobs, W.R., Jr., Larsen, M.H., Palmer, M.V., Nonnecke, B.J., Waters, R.W., Estes, M.D. (2009) Evaluation of Granulysin and Perforin as Candidate Biomarkers for Protection Following Vaccination with Mycobacterium bovis BCG or M. bovis ΔRD1. Transbound Emerg Dis 56: (6-7) 228-39. PMCID: PMC in process.

  142. Larsen, M., Biermann, K., Chen, B., Hsu, T., Sambandamurthy, V., Lackner, a., Ay, P.P., Didier, P. Huang, D., Shao, L.,  Huiyong, W., Letvin, N., Frothingham, R., Haynes, B., Chen, Z., Jacobs, W.R., Jr. (2009) Efficacy and Safety of Live Attenuated Persistent and Rapidly Cleared Mycobacterium tuberculosis Vaccine Candidates in Non-Human Primates. Vaccine 34:4709-4717 [Epub]. PMCID: PMC 3512200.

  143. Colangeli, R., Haq, A., Arcus, V.L., Summers, E., Magliozzo, R.S., McBride, A., Mitra, A.K., Radjainia, M., Khajo, a., Jacobs, W.R., Jr., Salgame, P., Alland, D. (2009) The Multifunctional Histone-Like Protein Lsr2 Protects Mycobacteria Against Reactive Oxygen Intermediates.  PNAS [early edition]. PMCID: PMC2657463.

  144. Zimmerman, D.M., Waters, W.R., Lyashcenko, K.P., Nonnecke, B.J., Armstrong, D.L., Jacobs, W.R., Jr., Larsen, M.H., Egan, E., Dean, G.A. (2009) Safety and immunogenicity of Mycobacterium tuberculosis DlysA DpanCD Vaccine in Domestic Cats Infecteed with Feline Immunodeficiency Virus. Clin Vaccine Immunol 16: (3) 427-429 [Epub]. PMCID: PMC 2650874.

  145. Chen,J., Kriakov,J., Singh,A., Jacobs,W.R., Besra,G.S., Bhatt,A., (2009) Defects in glycopeptidolipid biosynthesis confer phage 13 resistance in Mycobacterium smegmatis. PLoS Pathog 5 (4):e1000392.

  146. Ioerger,T.R., Koo,S., No,E.G., Chen,X., Larsen,M.H., Jacobs,W.R.,Jr., Pillay, M., Sturm, A.W., Sacchettini, J.C. (2009) Genome analysis of multi-and extensively-drug-resisant tuberculosis from KwaZulu-Natal, South Africa. PLoS One 4:(11)e778. PMCID:PMC2767505.

  147. Baughn, A., Garforth, S., Vilcheze, C., Jacobs, W.R.,Jr. (2009) An Anaerobic-Type α-Ketoglutarate Ferredoxin Oxidoreductase Completes the Oxidative Tricarboxylic Acid Cycle of Mycobacterium tuberculosis. PloS Path 5:(11)e1000662. PMCID PMC2773412.

  148. Waters, W.R., Palmer, M.V., Nonnecke, B.J., Thacker, T.C., Estes, D.M., Larsen, M.H., Jacobs, W.R., Jr., Andersen, P., McNair, Minion, F.C., Lyashchenko, K.P., Hewinson, R.G., Vordermeier, H.M., Sacco, R.E., (2009) Signal regularoty protein alpha (SIRPalpha) cells in the adaptive response to ESAT -6 CFP-10 protein of tuberculous mycobacteria.  PLos One 4:(7) e6414. PMCID: PMC2714177.

  149. Venkataswamy, M.M., Beana, A., Goldberg, M.F., Bricard, G., Im, J.S., Chan, J., Reddington, F., Besra, G.S., Jacobs, W.R., Jr., Porcelli, S.A. (2009) Incorporation of NKT cell-activating glycolipids enhances immunogenicity and vaccine efficancy of Mycobacterium bovis bacillus CAlmette-Guerin. J Immunol 183: (3) 1644-56. PMCID: PMC2719834.

  150. Banaei, N., Kincaid, E.Z., Lin, S.Y., Desmond, E., Jacobs, W.R.,Jr., Ernst, J.D. (2009) Lipoprotein processing is essential for resistance of Mycobacterium tuberculosis to malachite green. Antimicrob Agents Chemother (Epub).  PMCID: PMC 2737861.

  151. Kinhikar,A.G., Verma, I., Chandra, D., Singh, K.K., Weldingh, K., Andersen, P., Hsu, T., Jacobs, W.R., Jr., Laal, S. (2010) Potential role for ESAT6 in dissemination of M. tuberculosis via human lung epithelial cells. Mol. Microbiol. (75)1:92-106. PMCID PMC 2846543.

  152. Ranganathan, U.D., Larsen,M.H., Kim, J., Porcelli, S.A., Jacobs, W.R.,Jr., Fennelly, G.J. (2009) recombinant pro-apoptotic Mycobacterium tuberculosis generates CD8(+) T cell responses against human immunodeficiency virus type 1 Env and M. tuberculosis in neonatal mice. Vaccine10;28(1):152-161. PMCID PMC 2804991.

  153. Capyk, J.K., Kalscheuer,R., Stewart,G.R., Liu,J., Kwon,H., Zhao,R., Okamoto,S., Jacobs,W.R.,Jr., Eltis,L.D., Mohn,W.W. (2009) Mycobacterial cytochrome P450 125 (Cyp125) catalyzes the terminal hydroxylation of C27-steroids. J. Biol. Chem. 18:284(51):35534-42.  PMCID PMC2790983.

  154. Nascimento, I.P., Dias,W.O., Quintilio,W., Hsu,T., Jacobs, W.R.,Jr., Leite, L.C. (2009) Construction of an unmarked recombinant BCG expressing a pertussis antigen by auxotrophic complementation: Protection against Bordetella pertussis challenge in neonates. Vaccine.

  155. Gratraud, P., Huws, E., Falkard, B., Adjalley, S., Fidock, D.A., Jacobs, W.r., Jr., Baird, M.S., Vial, H., Kremer, L. (2009) Oleic Acid Biosynthesis in Plasmodium falciparum: characterization of the Stearoyl-CoA Desaturase and Validation as a Therapeutic Target. PLoS One. (4) 9:e6889. PMCID PMC 2731242.

  156. Qiu, L., Huang, D., Chen, C.Y., Wang, R., Shen, L., Shen, Y., Hunt, R., Estep, J., Haynes, B.F., Jacobs, W.R., Jr., Letvin, N., Du, G., Chen, Z. (2008) Severe Tuberculosis Induces Unbalanced Up-Regulation of Gene Networks and Overexpression of IL-22, MIP-1alpha, CCL27, IP-10, CCR4, CCR5, CXCR3, PD1, PDL2, IL-3 IFN-beta, TIM1, and TLR2 But Low Antigen-Specific Cellular Responses.  J. Infec Dis 198:1514-1519. PMCID: PMC 2884371

  157. Vilcheze, C., Av-Gay, Y., Attarian, R., Liu, Z., Hazbon, M.H., Colangeli, R., Chen, B., Liu, W., Alland, D., Sacchettini, J.C., Jacobs, Jr., W.R. (2008) Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis. Mol Microbiol69:1316-29. PMCID: PMC2628429.

  158. Arai, M., Sobou, M., Vilcheze, C., Baughn, A. Hashizume, H., Pruksakorn, P., Ishida, S.,Matsumoto, Ma., Jacobs.W.R.Jr.,Kobayashi, M. (2008) Halicyclamine A, a marine spongean alkaloid as a lead for anti-tuberculosis agent. Bioorg. Med. Chem. 16:6732-6. PMCID: PMC in process.

  159. Kumar, V., Loganathan, P., Sivaramakrishnan, G., Kriakov, J., Dusthakeer, A., Subramanyam, B., Chan, J., Jacobs, W.R., Jr., Paranji, Rama, N. (2008) Characterization of temperate phage Che12 and construction of a new tool for diagnosis of tuberculosis. Tuberculosis. PMCID: PMC 2678029.

  160. Mohamemohaideen, N.N., Palaninathan, S.K., Morin, P.M., Williams, B.J., Braunstein, M., Tichy, S.E., Locker, J., Russell, D.H., Jacobs, W.R., Jr., Sacchettini, J.C. (2008) Structure and Function of the Virulence-Associated High-Temperature Requirement A of Mycobacterium tuberculosis. Biochemistry 47 (23):6092-102 PMCID: PMC in process.

  161. Jayakumar, D., Jacobs, W.R., Jr., Narayanan, S. (2008) Protein Kinase E. of Mycobacterium tuberculosis Has a Role in the Nitric Oxide Stress Response and Apoptosis in a Human Macrophage Model of Infection. Cell Microbiol. 10:365-74. PMCID: PMC in process.

  162. Ojha, A.K., Baughn, A.D., Sambandan, D., Hsu, T., Trivellil, X., Guerardel, Y., Alahari, A., Kremer, L., Jacobs, W.R., Jr., Hatfull, G.F. (2008) Growth of Mycobacterium tuberculosis biofilms containing free mycolic acids and harboring drug tolerant bacteria. Mol. Microbiol. 69:164-74. PMCID: PMC2615189.

  163. Dao, D. N., K. Lawrence, T. Hsu, I. P. Nascimento, D. Roshevsky, S. S. Gurcha, G. S. Besra, J. Chan, S. A. Porcelli, and W. R. Jacobs (2008) Mycolic acid modification by the mmaA4 gene of M. tuberculosis modulates IL-12 production. PLoS Pathog 4:e1000081.pp.1-14. PMCID: PMC2390761.

  164. Mishra, A.K., Alderwich, L.J., Rittmann, D., Wang, C., Bhatt, A., Jacobs, W.R.,Jr., Takayama, K., Eggeling, L., Besra, G.S., (2008)  Identification of  a novel alpha (1-->6) mannopyranosyltransferase MptB from Corynebacterium glutamicum by deletion of a conserved gene, NCgl1505, affords a lipomannan- and lipoarabinomannan-deficient mutant. Mol Microbiol 68 (6): 1595-613. PMCID: PMC2440535.

  165. Im, J.S., Kang, T.J., Lee, S.B., Kim, C.H., Lee, S.H., Venkataswamy, M.M., Serfass, E.R., Chen, B., Illarionov, P.S., Besra, G.S., Jacobs, W.R., Jr., Chae, G.T., Porcelli, S.A. (2008) Alteration of the Relative Levels of iNKT Cell Subsets is Associated with Chronic Mycobacterial Infections. Clin. Immunol 127:214-224. PMCID: PMC2413133.

  166. Huang, D., Shen, Y., Qiu, L., Chen, C.Y., Shen, L., Estep, J., Hunt, R., Vasconcelos, D., Du, G., Qye, P., Lackner, A.A., Larsen, M.H., Jacobs, W.R., Haynes, B.F., Letvin, N.L., Chen, Z.W. (2008) Immune distribution and Localization of Phosphoantigen-Specific Vγ2VD2 T Cells in Lymphoid and Nonlymphoid Tissues in Mycobacterium tuberculosis Infection. Infect Immun 76:426-436. PMCID: PMC2223676.

  167. Gopalaswamy, R., Narayanan, S., Jacobs, W.R., Jr., Av-Gay, Y. (2008) Mycobacterium smegmatis Biofilm Formation and Sliding Motility are Affected by the Serine-Threonine Protein Kinase PknF. FEMS Microbiol. 278:121-127.PMCID: PMC in process.

  168. Banaiee, N., January, V., Barthus, C., Lambrick, M., RoDiti, D., Behr, M.A., Jacobs, W.R., Jr., Steyn, L.M. (2008) Evaluation of a Semi-Automated Reporter Phage Assay for Susceptibility Testing of Mycobacterium tuberculosis Isolates in South Africa. Tuberculosis 88:64-68. PMCID: PMC3677954..

  169. Reddy, M.C.M., Gokulan, K., Jacobs, W.R., Jr., Ioerger, T.R., Sacchettini, J.C. (2008) Crystal structure of Mycobacterium tuberculosis LrpA, a Leucine-Responsive Global Regulator Associated with Starvation Response.  Protein Science 17:159-170. PMCID: PMC2144582.

  170.   Bueno, S.M., Gonzalez, P.A., Cautivo, K.M., Mora, J.E., Leiva, E.D., Tobar, H.E., Fennelly, G.J., Eugenin, E.A., Jacobs, W.R., Jr., Riedel, C.A., Kalergis, A.M. (2008) Protective T Cell Immunity Against Respiratory Syncytial Virus is Efficiently Induced by Recombinant BCG. PNAS 105:20822-20827. PMCID: PMC2634951.

  171. Yu, M., Kumar, T.R., Nkrumah, L.J., Coppi, A., Retzlaff, s., Li, C.D., Kelly, B.J., Moura, P.A., Lakshmanan, Freundlich, J.s., Valderramos, J.C., Vilcheze, C., Siedner, M., Tsai, J.H., Falkard, B., Sidhu, A.B., Purcell, L.A., Gradtraud, P, Kremer, L, Waters, A.P., Schiehser, G., Jacobus, D.P., Janse, C.J., Ager, A., Jacobs,   W.R., Jr., Sacchettini, J.C., Heussler, V., Sinnis, Pl, Fidock, D.A. (2008) The Fatty Acid Biosynthesis Enzyme FabI Plays a Key Role in the Development of Liver-Stage Malarial Parasites. Cell Host Microbe 4:567-578. PMCID: PMC2646117.

  172. Bhatt, K., S. S. Gurcha, A. Bhatt, G. S. Besra, and J. Jacobs, W.R. (2007) Two Polyketide Synthase-Associated Acyl Transferases are Required for Sulfolipid Biosynthesis in Mycobacterium tuberculosis. Microbiol. 153:513-520. PMCID: PMC in process.

  173. Waters, W.R., Palmer, M.V., Nonnecke, B.J., Thacker, T.C., Scherer, C.F., Estes, D.M., Jacobs, W.R., Jr., Glatman-Freeman, A., Larsen, M.H. (2007) Failure of a Mycobacterium tuberculosis DRD1 DpanCD Double Deletion Mutant in a Neonatal Calf Aerosol M. bovis Challenge Model:  Comparisons to Responses Elicited by M. bovis bacilli Calmette Guerin. Vaccine 25:7832-7840. PMCID: PMC in process.

  174. Banaiee, N., Jacobs, W.R., Jr., Ernst, J. (2007) LspA-Independent Action of Globomycin on Mycobacterium tuberculosis. J. Antimicrob. Chemother. 60:414-416. PMCID: PMC in process.

  175. Al-Sayyed, B., Piperdi, S., Yuan, X., Li, A., Besra, G.S., Jacobs, W.R., Jr., Casadevall, A., Glatman-Freeman, A. (2007) Monoclonal Antibodies to Mycobacterium tuberculosis CDC 1551 Reveal Subcellular Localization of MPT51. Tuberculosis  87:489-97. PMCID: PMC2475595.

  176. Velmurugan, K., Chen, B., Miller, J.L., Azogue, S., Gurses, S., Hsu, T., Glickman, M., Jacobs, W.R., Porcelli, S.A., Briken, V.  (2007) Mycobacterium tuberculosis nuoG Is A Virulence Gene That Inhibits Apoptosis of Infected Host Cells. PLoS Pathog. 3:e110 [Epub ahead of print] PMCID: PMC1924871.

  177. Zimhony, O., Vilcheze, C., Arai, M., Welch, J., Jacobs, W., Jr. (2007)  Pyrazinoic Acid and its n-Propyl Ester Inhibit Fatty Acid Synthase I in Replicating Tubercle Bacilli. Antimicrobiol. Agents Chemother. 51:752-754. PMCID: PMC1797748.

  178. Ngo, S.C., Zimhony, O., Chung, W.J., Sayahi, H., Jacobs, W., Jr. and Welch, J.T. (2007) Inhibition of Isolated  Mycobacterium tuberculosis Fatty Acid Synthase I by Pyrazinamide Analogs. Antimicrobiol. Agents Chem. 51:2430-2435. PMCID: PMC1913273.

  179. Hinchey, J., Lee, S., Jeon, B.-Y., Basaraba, R.J., Venkataswamy, M.M.,  Chen, B., Chan, J., Braunstein, M., Orme, I.M., Derrick, S.C., Morris, S.L., Jacobs, Jr., W.R. and Porcelli, S.A. (2007) Enhanced Priming of Adaptive Immunity by A Proapoptotic Mutant of Mycobacterium tuberculosis. J Clin Invest. 117:2279-2288. PMCID: PMC1934588.

  180. Bhatt, A., Molle, V., Besra, G.S., Jacobs, W.R., Jr., Kremer, L. (2007) The Mycobacterium tuberculosis FAS-II Condensing Enzymes:  Their Role in Mycolic Acid Biosynthesis, Acid-Fastness, Pathogenesis and in Future Drug Development. Mol. Microbiol. 64:1442-1454. PMCID: PMC in process.

  181. Freundlich, J.S., Wang, F., Tsai, H.C. Kuo, M., Sheih, H.M., Anderson, J.W., Nkrumah, L.J., Valderramos, J.C., Yu, M., Kumar, T.R., Valderramos, S.G., Jacobs, W.R., Schiehser, G.A., Jacobus, D.P., Fidock, D.A., Sacchettini, J.C. (2007) X-ray Structural Analysis of Plasmodium falciparum Enoyl Acyl Carrier Protein Reductase as a Pathway Toward the Optimization of Triclosan Antimalarial Efficacy. J. Biol. Chem. 282:25436-25444. PMCID: PMC in process.

  182. Colangeli, R., Helb, D., Vilcheze, C., Hazbon, M.H., Lee, C.G., Safi, H., Sayers, B., Sardone, I., Jones, M.B., Fleischmann, R.D., Peterson, S.N., Jacobs, W.R., Jr., Alland, D. (2007) Transcriptional Regulation of Multi-Drug Tolerance and Antibiotic-Induced Responses by the Histone-Like Protein Lsr2 in M. tuberculosisPLoS Pathog. 3:e87. PMCID: PMC1894825.

  183. Pinto, R., Harrison, J.S., Hsu, T., Jacobs, W.R., Jr., Leyh, T.S. (2007) Sulfite Reduction in Mycobacteria. J. Bacteriol. 189:6714-6722. PMCID: PMC2045171.

  184. Mo, Y., Quanquin, N.M., Vecino, W.H., Raganathan, U.D., Tesfa, L., Boom, W., Derbyshire, G.M., Letvin, N.L., Jacobs, W.R., Jr., Fennelly, G.J. (2007) Genetic Alteration of Mycobacterium smegmatis to Improve Mycobacterium-Mediated Transfer of Plasmid DNA into Mammalian Cells and DNA immunization. Infect. Immun. 75:4804-4816. PMCID: PMC2044538.

  185. Glover, R. T., J. Kriakov, S. J. Garforth, and W. R. Jacobs, Jr. ( 2007) The Two-Component Regulatory System senX3-regX3 Regulates Phosphate Dependent Gene Expression in Mycobacteria. J Bacteriol 189:5495-5503. PMCID: PMC1951828.

  186. Bhatt, A., N. Fujiwara, K. Bhatt, S. Gurcha, L. Kremer, B. Chen, J. Chan, S. Porcelli, K. Kobayashi, G. Besra, and J. Jacobs, W.R. (2007) Deletion of kasB in Mycobacterium tuberculosis Causes a Loss of Acid-Fastness and Subclinical Latent Tuberculosis in Immunocompetent Mice. PNAS, USA 104:5157-5162. PMCID: PMC1829279.

  187. Wang, F., Langley, R., Gulten, G., Dover, L.G.,  Besra, G.S., Jacobs, Jr., W.R. and Sacchettini, J.C. (2007) Mechanism of Thioamide Drug Action against Tuberculosis and Leprosy J. Exp. Med. 204:73-78. PMCID: PMC2118422.

  188. Hovav, A.H., Cayabyab, M.J., Panas, M.W., Santra, S., Greenland, J., Geiben, R., Haynes, B.F., Jacobs, W.R., Jr., Letvin, N.L. (2007) Rapid Memory CD8+ T-Lymphocyte Induction Through Priming with Recombinant Mycobacterium smegmatis. J. Virol. 81:74-83. PMCID: PMC1797252.

  189. Derrick, S., T. Evering, V. K. Sambandamurthy, K. V. Jalapathy, T. Hsu, B. Chen, M. Chen, R. Russell, A. P. Junqueira-Kipnis, I. M. Orme, S. Porcelli, J. Jacobs, W.R., and S. L. Morris (2007) Characterization of the Protective T Cell Response Generated in CD4 Deficient Mice By A Live Attenuated Mycobacterium tuberculosis Vaccine. Immunology 120:192-206. PMCID: PMC2265854.

  190. Yu, J.S., Peacock, J.W., Jacobs, W.R., Jr., Frothingham, R., Letvin, N.L., Liao, H.X., Haynes, B.F. (2007) Recombinant Mycobacterium bovis bacillus Calmette-Guerin elicits human immunodeficiency virus type 1 envelope-specific T lymphocytes at mucosal sites. Clin Vaccine Immunol 14 (7) 886-93. PMCID: PMC1951062.

  191. Yu, J. S., J. W. Peacock, S. Vanleeuwen, T. Hsu, W. R. Jacobs, Jr., M. J. Cayabyab, N. L. Letvin, R. Fronthingham, H. F. Staats, H. X. Liao, and B. F. Haynes (2006) Generation of Mucosal Anti-Human Immunodeficiency Virus Type 1 T Cell Responses by Recombinant Mycobacterium smegmatis. Clin Vaccine Immunol. 13:1204-1211. PMCID: PMC1656549.

  192. Lee, S., Jeon, B.-Y., Bardarov, S., Chen, M., Morris, S.L. and Jacobs, W.R., Jr. (2006) Protection Elicited by Two Glutamine Auxotrophs of Mycobacterium tuberculosis and in vivo Growth Phenotypes of the Four Unique Glutamine Synthetase Mutants in a Murine Model. Infec. Immun. 74:6491-6495. PMCID: PMC1695480.

  193. Junqueira-Kipnis, A.P., Basaraba, R.J., Gruppo, V., Palanisamy, G., Turner, O.C., Hsu, T., Jacobs, W.R., Jr., Fulton, S.A., Reba, S.M., Boom, W.H., Orme, I.M. (2006) Mycobacteria Lacking the RD1 Region Do Not Induce Necrosis in the Lungs of Mice Lacking Interferon-γ. Immunology 119:224-231. PMCID: PMC1782352.

  194. Vilchèze, C., Wang, F., Arai, M., Hazbòn, M.H., Colangeli, R., Kremer, L., Weisbrod, T.R., Alland, D., Sacchettini, J.C., Jacobs, W.R., Jr. (2006) Transfer of a Point Mutation in Mycobacterium tuberculosis inhA Resolves the Target of Isoniazid. Nat. Med. 12:1027-1029.PMCID: PMC in process.

  195. Nkrumah, L., Moura, P., Ghosh, P., Hatfull, G., Jacobs, W.R. Jr., Fidock, D.A. (2006)  Efficient Site-Specific Integration in Plasmodium falciparum Chromosomes Medicated by Mycobacteriophage BxB1 Integrase. Nat. Meth. 3:615-621. PMCID: PMC2943413.

  196. Sambandamurthy, V.K., Derrick, S.C., Hsu, T., Chen, B., Larsen, M.H., Jalapathy, K.V., Chen, M., Kim, J., Porcelli, S.A., Chan, J., Morris, S.L., Jacobs, W.R. Jr. (2006)  Mycobacterium tuberculosis DRD1 DpanCD: A Safe and Limited Replicating Mutant Strain that Protects Immunocompetent and Immunocompromised Mice Against Experimental Tuberculosis. Vaccine 11:6309-6320. PMCID: PMC in process.

  197. Perez, J., Garcia, R., Bach, H., de Waard, J.H., Jacobs, W. R., Jr., Av-Gay, Y., Bubis, J., Takiff, H.E. (2006) Mycobacterium tuberculosis Transporter MmpL7 is a Potential Substrate for Kinase PknD. Biochem. Biophy. Res. Comm. 348:6-12. PMCID: PMC in process.

  198. Hatfull, G.F., Pedulla, M.L., Jacobs-Sera, D., Cichon, P.M., Foley, A., Ford, M.E., Gonda, R.M., Houtz, J.M., Hryckowian, A.J., Kelchner,  V., Namburi, S. Pajcini, K.J. Popovich, M., Schleicher, D.S., Simanek, B.R., Smith, A.S., Zdanowica, G.M., Kumar, V., Peebles, C.L., Jacobs, W.R. Jr., Lawrence, J.G., Hendrix, R.W. (2006) Exploring the Mycobacteriphage Metaproteome:  Phage Genomics as an Educational Platform. PLos Genetics 2:e92:0835-0847. PMCID: PMC1485703.

  199. Aravindhan, V., Narayanan, S., Gautham, N., Prasad, V., Kannan, P., Jacobs, W.R., Jr., Narayanan, P.R. (2006) T-h-2 Immunity and CD3+ CD45RBlow-Activated T Cells in Mice Immunized with Recombinant Bacillus Calmette Guerin Expressing HIV-1 Principal Neutralizing Determinant Epitope. FEMS Immunol. Med. Microbiol. 47:45-55. PMCID: PMC in process.

  200. Roy, E., De Silva, A.D., Sambandamurthy, V.K., Clark, S.O., Stavropoulos, E., Jacobs, W.R., Jr., Chan, J., Williams, A., Colston, M.J., Tascon, R.E. (2006) Induction of High Levels of Protective Immunity After Vaccination Using Dendritic Cells Infected with Auxotrophic Mutants of Mycobacterium tuberculosis. Immunol. Lett. 103:196-199. PMCID: PMC in process.

  201.  Morbidoni, H.R., Vilcheze, C., Kremer, L., Bittman, R., Sacchettini, J.C., Jacobs, W.R., Jr. (2006) Dual Inhibition of Mycobacterial Fatty Acid Biosynthesis and Degradation by 2-Alkynoic Acids.  Chem. Biol. 13:297-307. PMCID: PMC in process.

  202. Tufariello, J.A.M., Mi, K., Xu, J., Manabe, Y.C., Kesavan, A.K., Drumm, J., Tanaka, K., Jacobs, W.R. Jr., Chan, J. (2006) Deletion of the Mycobacterium tuberculosis Resuscitation-Promoting factor Rv1009 Gene Results in Delayed Reactivation from Chronic Tuberculosis. Infec.  Immun. 74:2985-2995. PMCID: PMC1459759.

  203. Govan, V.A., Christensen, N.D., Berkower, C., Jacobs, W.R., Jr, and Williamson, A.-L. (2006) Immunisation With Recombinant BCG Expressing the Cottontail Rabbit Papillomavirus (Crpv) L1 Gene Provides Protection From Crpv Challenge. Vaccine  24:2087-2093. PMCID: PMC in  process.

  204. Rao, V., Gao, F., Chen, B., Jacobs, W.R.,Jr. Glickman, M.S. (2006) Trans Cyclopropanation of Mycolic Acids on Trehalose Dimycolate Suppresses M. tuberculosis Induced Inflammation and Virulence. J. Clin. Invest. 116:1660-1667. PMCID: PMC1464906.

  205. Banaiee, N., Kincaid, E.Z., Buchwald, U., Jacobs, W.R. Jr., Ernst, J.D. (2006) Potent Inhibition of Macrophage Responses to IFN-g by Live Virulent Mycobacterium tuberculosis is Independent of mature Mycobacterial Lipoproteins but Dependent on TLR21. J. Immunol. 176:3019-3027. PMCID: PMC in process.

  206. Freundlich, J.S., Yu,M., Lucumi, E., Kuo, Tsai, H.C., M., Valderramos, J.C., Karagyozov, L, Jacobs, W.R. Jr., Schiehser, G. A., Fidock, D. A., Jacobus, D. P., Sacchettini, J.C. (2006)  Synthesis and Biological Activity of Diaryl Ether Inhibitors of Malarial Enoyl Acyl Carrier Protein Reductase. Part 2: 2’ –Substituted Triclosan Derivatives. Bioorganic & Medicinal Chemistry Letters. 16: 2163-2169. PMCID: PMC in process.

  207. Schwebach, J. R., and W. R. Jacobs, Jr. (2006) Phage Finding Using Mycobacteria:  A Secondary School of Undergraduate Research Module with the Potential to Gain Scientific Authorship. The American Biology Teacher  68:482-490. PMCID: PMC n/a

  208. Banaiee, N., Jacobs, W.R., Jr. and Ernst, J. (2006) Regulation of Mycobacterium tuberculosis whiB3 in the mouse lung and macrophages.Infect Immun. 74:6449-6457. PMCID: PMC1695489.

  209. Cayabyab, M.J., Hovav, A.H., Hsu, T., Krivulka, G.R., Lifton, M.A., Gorgone, D.A., Fennelly, G.J., Haynes, B.F., Jacobs, W.R., Jr., Letvin, N.L. (2006) Generation of CD8+ T-cell responses by a Recombinant Nonpathogenic Mycobacterium smegmatis Vaccine Vector expressing Human Immunodeficiency virus type 1 Env. J. Virol. 80:1645-1652. PMCID: PMC1367151.

  210. Ojha, A., Anand, M.,  Bhatt, A.,  Kremer, L., Jacobs, W.R.,Jr. Hatfull, G.F. (2005) GroEL1: A  Dedicated Chaperone Involved in Mycolic Acid Biosynthesis during Biofilm formation in Mycobacteria. Cell 123:861-873. PMCID: PMC in process.

  211. Bhatt, A., Kremer, L., Dai, A.Z., Sacchettini, J.C., and Jacobs, W.R.,Jr..  (2005) Conditional Depletion of KasA, a Key Enzyme of Mycolic Acid biosynthesis, Leads to Myobacterial Cell Lysis.  J. Bacteriol. 187:7596-7606. PMCID: PMC1280301.

  212. Colangeli, R., Helb, D., Sridharan, S., Sun, J., Varma-Basil, M., Hazbon, M.H., Hom, D., Harbacheuski, R., Megjugorac, N.J., Jacobs, W.R., Jr., Holzenberg, A., Sacchettini, J.C. and Alland, D. (2005) The Mycobacterium tuberculosis iniA Gene is Essential for Activity of an Efflux Pump that Confers Drug Tolerance to Both Isoniazid and Ethambutol.  Mol. Microbiol. 55:1829-1840. PMCID: PMC in process.

  213. Vilcheze, C., Weisbrod, T.R., Chen, B., Kremer, L., Hazbon, M.H., Wang, F., Alland, D., Sacchettini, J.C., Jacobs, W.R., Jr. (2005) Altered NADH/NAD+ Ratios Mediate Coresistance to Isoniazid and Ethionamide in Mycobacteria. Antimicrobiol. Agents Chem. 49:708-720. PMCID: PMC547332.

  214. Sambandamurthy V.K., Derrick, S.C., Jalapathy, K.V., Chen, B., Russell, R.G., Morris, S.L. and Jacobs, W.R., Jr.(2005) Long-Term Protection Against Tuberculosis Following Vaccination with a Severely Attenuated Double Lysine and Pantothenate Auxotroph of Mycobacterium tuberculosis Infec. Immun. 73:1196-1203. PMCID: PMC547051.

  215. Sambandamurthy, V.K., Jacobs, W.R. Jr. (2005)  Live  attenuated mutants of Mycobacterium tuberculosis as candidate vaccines against tuberculosis. Micro.  Infect. 7:955-961. PMCID: PMC in process.

  216. Freundlich, J.S., Anderson, J.W., Sarantakis, D., Shieh, H.M., Yu, M., Valderramos, J.C., Lucumi, E., Kuo, M., Jacobs, W.R. Jr., Fidock, D.a., Schiehser, G.A., Jacobus, D.P., Sacchettini, J.C. (2005) Synthesis, Biological activity, and X-ray Crystal Structural Analysis of Diaryl Ether Inhibitors of Malarial Enoyl Acyl Carrier Protein Reductase.  Part 1: 4’-Substituted Triclosan Derivatives. Bioorganic & Medicinal Chemistry Letters. 15:5247-5252. PMCID: PMC in process.

  217. Zimhony, O., Vilchèze, C. and Jacobs, W.R., Jr. (2004) Characterization of a Recombinant Mycobacterium smegmatis Strain Containing a Fatty Acid Synthase I Gene Replaced with the M. tuberculosis Homologue. J. Bacteriol., 186:4051-4055. PMCID: PMC421601.

  218. Tufariello, J.M., Jacobs, W.R., Jr., Chan, J. (2004) Individual Mycobacterium tuberculosis Resuscitation-Promoting Factor Homologues Are Dispensable for Growth In Vitro and In Vivo. Infec. Immun. 72:515-526. PMCID: PMC343985.

  219. Hisert, K.B., Kirksey, M.A., Gomez, J.E. Sousa, A.O., Cox, J.S., Jacobs, W.R., Jr., Nathan, C.F., McKinney, J.D. (2004) Identification of Mycobacterium tuberculosis counterimmune (cim) mutants in immunodeficient mice by differential screening. Infect. Immun. 72:5315-5321. PMCID: PMC517420.

  220. Vecino, W.H., Quanquin, N.M., Martinez-Sobrido, L., Fernandez-Sesma, A., Garcia-Sastre, A., Jacobs, W.R., Jr., Fennelly, G.J. (2004) Mucosal Immunization with Attenuated Shigella flexneri Harboring an Influenza Hemagglutinin DNA Vaccine Protects Mice Against A Lethal Influenza Challenge. Virology 325:192-199. PMCID: PMC in process.

  221. Sampson, S.L., Dascher, C.C., Sambandamurthy, V.K., Russell, R.G., Jacobs, W.R., Jr., Bloom, B.R., and Hondalus, M.K. (2004) Protection Elicited By A Double Leucine And Pantothenate Auxotroph Of Mycobacterium Tuberculosis In Guinea Pigs, Infec. Immun., 72:3031-3037. PMCID PMC387862.

  222. Matsunaga, I., Bhatt, A., Young, D.C., Chen, T.-Y., Eyles, S.J., Besra, G.S., Briken, V., Porcelli, S.A., Costello, C.E., Jacobs, W.R., Jr., Moody, D.B. (2004) Mycobacterium tuberculosis pks 12 Produces a Novel Polyketide Presented by CD1c to T Cell.  J. Exp. Med. 200:1559-1569. PMCID: PMC2211992.

  223. Lee, S., Kriakov, J., Vilcheze, C., Dai, Z., Hatfull, G.F., Jacobs, W.R., Jr.  (2004) Bxz1, a New Generalized Transducing Phage for Mycobacteria. FEMS Microbiol. 241:271-276. PMCID: PMC in process.

  224. Glatman-Freedman, A., Casadevall, A., Dai, Z., Jacobs, W.R., Jr., Li, A., Morris, S.L., Navoa, J.A.D., Piperdi, S., Robbins, J.B., Schneerson, R., Schwebach, J.R., Shapiro, M. (2004) Antigenic Evidence of Prevalence and Diversity of Mycobacteria tuberculosis Arabinomannan. J. Clin. Microbiol. 42:3225-3231. PMCID: PMC446310.

  225. Dao, D.N., Kremer, L., Guerardel, Y., Molano, A., Jacobs, W.R., Jr., Porcelli, S.A., Briken, V. (2004) Mycobacterium tuberculosis  Lipomannan Induces Apoptosis and Interleukin-12 Production in Macrophages. Infec. Immun. 72:2067-2074. PMCID: PMC375177.

  226. Hazbón, M.H., Ferro, B.E., Guarín, Rodriguez, A.L., Riska, P.F., Jacobs, W.R., Jr. (2003) Photographic and Luminometric Detection of Luciferase Reporter Phages for Drugs Susceptibility Testing of Clinical Mycobacterium tuberculosis Isolates.  J. Clin. Microbiol., 41:4865-4869. PMCID PMC254324.

  227. Ohno, H., Zhu, G., Mohan, V.P., Chu, D., Kohno, S., Jacobs, W.R., Jr., Chan, J. (2003) The Effects of Reactive Nitrogen Intermediates on Gene Expression of Mycobacterium tuberculosis. Cell. Microbiol. 5:637-648. PMCID: PMC in process.

  228. Hsu,T., Hingley-Wilson, S., Chen, B., Morin, P.M., Marks, C.B., Goulding, C., Gingery, M., Eisenberg, Russell, R.G., Derrick, S.C., Collins, F.M., Morris, S.L., King, C.H. and Jacobs, W.R. Jr. (2003) The Primary Mechanism of Attenuation of bacille Calmette-Guérin Is a Loss of Secreted Lytic Function Required for Invasion of Lung Interstitial Tissue. PNAS, USA, 100:12420-12425. PMCID: PMC218773.

  229. Banaiee, N., Bobadilla-del-Valle, M., Riska, P.F., Bardarov, S., Jr., Small, P.M., Ponce-de-Leon, A., Jacobs, W.R., Jr., Hatfull, G.F., Sifuentes-Osornio, J. (2003) Rapid Identification and Susceptibility Testing of Mycobacterium tuberculosis from MGIT Cultures with Luciferase Reporter Mycobacteriophages.  J. Med. Microbiol. 52:557-561. PMCID: PMC in process.

  230. Gokulan, K., Rupp, B., Pavelka, M., Jacobs, W., Jr., Sacchettini, J.C. (2003) Crystal Structure of Mycobacterium tuberculosis Diaminopimelate Decarboxyalse, an Essential Enzyme in Bacterial Lysine Biosynthesis. J. Biol. Chem. 278:18588-18596. PMCID: PMC in process.

  231. Kuo, M.R., Morbidoni, H.R. Alland, D., Sneddon, S.F., Gourlie B.B., Staveski, M.M., Leonard, M., Gregory, J.S., Janjigian, A.D., Yee, C., Kreiswirth, B., Iwamoto, H., Perozzo, R., Jacobs, W.R., Jr., Sacchettini, J.C., Fidock, D.A. (2003) Targeting Tuberculosis and Malaria Through Inhibition of Enoyl Reductase:  Compound Activity and Structural Data. J. Biol. Chem. 278:20851-20859. PMCID: PMC in process.

  232. Kremer, L., Dover, L.G., Morbidoni, H.R., Vilcheze, C., Maughan, W.N., Baulard, A., Tu, S.-C., Honore, N., Deretic, V., Sacchettini, J.C., Locht, C., Jacobs, W.R., Jr. and Besra, G.S. (2003) Inhibition of InhA Activity, but not KasA Activity, Induces Formation of a KasA-Containing Complex in Mycobacteria. J. Biol. Chem. 278:20547-20554. PMCID: PMC in process.

  233. Sharma, V., Arockiasamy, A., Ronning, D.R., Savva, C.G., Holzenburg, A., Braunstein, M., Jacobs, W.R., Jr. and Sacchettini, J.C. (2003) Crystal Structure of M. tuberculosis SecA, a Preprotein Translocating ATPase. Proc. Natl. Acad. Sci., USA 100:2243-2248. PMCID: PMC151325.

  234. Pedulla, M.L., Ford, M.E., Houtz, J.M., Karthikeyan, T., Wadsworth, C.J., Lewis, J.A., Jacobs-Sera, D., Falbo, J., Gross, J., Pannunzio, N., Brucker, W., Kumar, V., Kandasamy, J., Keenan, L., Bardarov, S., Jr., Kriakov, J., Lawrence, J.G., Jacobs, W.R., Jr., Hendrix, R.W. and Hatfull, G.F. (2003)  Origins of Highly Mosaic Mycobacteriophage Genomes. Cell 113:171-182. PMCID: PMC in process.

  235. Pavelka, M.S., Jr., Chen, B., Kelley, C.L., Collins, F.M. and Jacobs, W.R., Jr. (2003) Vaccine Efficacy of a Lysine Auxotroph of M. tuberculosis. Infec. Immun. 71:4190-4192. PMCID: PMC 161997.

  236. Braunstein, M., Espinosa, B., Chan, J., Belisle, J.T. and Jacobs, W.R., Jr. (2003) Sec A2 Functions in the Secretion of Superoxide Dismutase A and in the  Virulence of Mycobacterium tuberculosis. Mol. Microbiol. 48:453-464. PMCID: PMC in process.

  237. Otero, J., Jacobs, W.R., Jr. and Glickman, M.S. (2003) Efficient Allelic Exchange and Transposon Mutagenesis in Mycobacterium avium Using Specialized Transduction. Appl. Environ. Microbiol. 69:5039-5044. PMCID: PMC194949.

  238. Kriakov, J and Jacobs, W.R., Jr. (2003) Identification of a Cryptic Alkaline Phosphatase in Mycobacterium smegmatis. J. Bacteriol. 185:4983-4991. PMCID: PMC166462.

  239. Consaul, S.A., Jacobs, W.R., Jr. and Pavelka, M.S., Jr. (2003) Extragenic Suppression of the Requirement for Diaminopimelate in diaminopimelate auxotrophs Mycobacterium smegmatis. FEMS Micro. Letts, 225:131-135. PMCID: PMC in process.

  240. Bhatt, A. and Jacobs, W.R., Jr. (2003) Conjugal Rites of Mycobacteria. Nat. Genetics, 34:3-4. PMCID: PMC in process.

  241. Bardarov, Jr.,S, H. Dou, K. Eisenach, N. Banaiee, Y. Su, G.F. Hatfull, J. Chan, W.R. Jacobs, Jr. and P.F. Riska (2002) Detection and drug-susceptibility testing of  M. tuberculosis from sputum samples using luciferase reporter phage: Comparison with the Mycobacteria Growth Indicator Tube (MGIT) system, Diagnostic Microbiology & Infectious Disease 45:53-61. PMCID: PMC in process.

  242. Sambandamurthy, V.K., Wang X., Chen, B, Russell, R.G., Derrick, S., Collins, F.M., Morris, S.L. and Jacobs, W.R., Jr. (2002) A Pantothenate Auxotroph of Mycobacterium tuberculosis is Highly Attenuated and Protects Mice Against Tuberculosis. Nat. Med. 8:1171-1174. PMCID: PMC in process.

  243. McAdam, R.A., Quan, S., Smith, D.A., Bardarov, S., Betts, J.C., Cook, F.C., Hooker, E.U., Lewis, A., Woollard, P., Everett, M.J., Lukey, P.T., Bancroft, G.J., Jacobs, W.R. and Jr. and Duncan, K. (2002) Characterization of a Mycobacterium tuberculosis H37Rv Transposon Library Reveals Insertions in 351 ORFs and Mutants with Altered Virulence. Microbiology 148:2975-2986. PMCID: PMC in process.

  244. Bardarov, S., Bardarov, S. Jr., Pavelka, M. Jr., Sambandamurthy, V., Larsen, M., Tufariello, J., Chan, J., Hatfull, G. and Jacobs, W.R. Jr. (2002) Specialized Transduction:  An Efficient Method for Generating Marked and Unmarked Targeted Gene Disruptions in Mycobacterium tuberculosis, M. bovis BCG, and M. smegmatis. Microbiology 148:3007-3018.PMCID: PMC in process.

  245. Fleischmann, R.D., Alland, D., Eisen, J., Carpenter, L., White, O., Peterson, DeBoy, R., Dodson, R., Gwinn, M., Haft, D., Hickey, E., Kolonay, J.F., Nelson, W.C., Umayam, L.A., Ermolaeva, M., Salzberg, S.L., Delcher, A., Utterback, T., Weidman, J., Khouri, H., Gill, J., Mikula, A., Bishai, W., Jacobs, Jr., W.R., Venter, J.C. and Fraser, C.M. (2002) Whole Genome Comparison of Mycobacterium tuberculosis Clinical and Laboratory Strains.  J. Bacteriol. 184:5479-5490. PMCID: PMC 135346.

  246. Larsen, M.H., Vilcheze, C., Kremer, L., Besra, G.S., Parsons, L., Salfinger, M., Heifets, L, Hazbon, M.H., Alland, D., Sacchettini, J.C. and Jacobs, W.R.,Jr.  (2002) Overexpression of InhA, but not kasA, Confers Resistance to Isoniazid and Ethionamide in Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis. Mol. Microbiol. 2:453-466.PMCID: PMC in process.

  247. Schwebach, J.R., Chen, B., Glatman-Freedman, A., Casadevall, A., McKinney, J., Harb, J.L., McGuire, P.J., Barkley, W.E., Bloom, B.R. and Jacobs, W.R. Jr. (2002) Infection of Mice with Aerosolized Mycobacterium tuberculosis:  Use of a Nose-Only Apparatus for Delivery of Low Inocula and Design of an Ultra-Safe Facility. Appl. Environ. Microbiol. 68:4646-4649. PMCID: PMC124060.

  248. Vecino, W.H., Morin, P., Jacobs, W.R., Jr. and Fennelly, G.J. (2002) Mucosal DNA vaccination with highly attenuated Shigella is superior to attenuated Salmonella and comparable to intramuscular DNA vaccination of T cells against HIV. Immunol. Lett. 82:197-204. PMCID: PMC in process.

  249. Wooff, E., Michell, S.L., Gordon, S.V., Chambers, M.A., Bardarov, S., Jacobs, W.R., Jr., Hewinson, R.G. and Wheeler, P.R. (2002) Function genomics reveals the sole sulphate transporter of the Mycobacterium tuberculosis complex and its relevance to the acquisition of sulphur in vivo.  Mol. Microbiol. 43:653-663. PMCID: PMC in process.

  250. Steyn, A.J., Collins, D.M., Hondalus, M.K., Jacobs, W.R., Jr, Kawakami, R.P. and Bloom, B.R. (2002) Mycobacterium tuberculosis WhiB3 Interacts With RpoV to Affect Host Survival But Is Dispensable for in vivo Growth. Proc. Natl. Acad. Sci.USA  99:3147-3152. PMCID: PMC122487.

  251. Huang, C.-C., Smith, C.V., Glickman, M.S., Jacobs, W.R., Jr. and Sacchettini, J.C. (2002) Crystal Structures of Mycolic Acid Cyclopropane Synthases from Mycobacterium tuberculosis. J. Biol. Chem. 277:11559-11569. PMCID: PMC in process.

  252. Perozzo, R., Kuo, M., Singh, S., A.B., Valiyaveettil, J.T., Bittman, R., Jacobs, W.R., Jr., Fidock, D.A. and Sacchettini, J.C.  (2002) Structural Elucidation of the Specificity of the Antibacterial Agent Triclosan for Malarial Enoyl Acyl Carrier Protein Reductace.  J. Biol. Chem. 277:13106-13114. PMCID: PMC in process.

  253. Brennan, M.J., Delogu, G., Chen, Y., Bardarov, S., Kriakov, J., Alavi, M. and Jacobs, W.R., Jr. (2001) Evidence that a Mycobacterial PE_PGRS Protein Cell Surface Constituents that Influence Interactions with Other Cells.  Infect. Immun.69:7326-7333. PMCID: PMC98818.

  254. Banaiee, N., Bobadilla-del-Valle, M., Bardarov, S., Riska, P., Small, P.M., Ponce-de-Leon, A., Jacobs, W.R., Jr., Hatfull, G.F. and Sifuentes-Osornio, J. (2001) Luciferase Reporter Mycobacteriophages for Detection, Identification, and Antibiotic Susceptibility Testing of Mycobacterium tuberculosis in Mexico. J. Clin. Microbiol. 39:3883-3888. PMCID: PMC88459.

  255. Raman, S., Song, T., Puyang, X., Bardarov, S., Jacobs, W.R., Jr. and Husson, R.N. (2001) The Alternative Sigma Factor SigH Regulates Major Components of the Oxidative and Heat Stress Responses in Mycobacterium tuberculosisJ. Bacteriol. 183:6119-6125. PMCID: PMC99691.

  256. Schwebach, J.R., Jacobs, W.R., Jr. and Casadevall, A. (2001) Sterilization of Mycobacterium tuberculosis Samples by Antimicrobial Fixation in the BSL-3 Laboratory. J. Clin. Micrbiol. 39:769-771. PMCID: PMC87817.

  257. Braunstein, M., Brown, A.M., Kurtz, S. and Jacobs, W.R., Jr. (2001) Two Non-Redundant SecA Homologues Function in Mycobacteria.  J. Bacteriol. 183:6979-6990. PMCID: PMC95544.

  258. Glickman, M.S., Cahill, S.M. and Jacobs, W.R., Jr. (2001) The Mycobacterium tuberculosis cmaA2 Gene Encodes a Mycolic Acid trans Cyclopropane Synthetase.  J. Biol. Chem. 276:2228-2233. PMCID: PMC in process.

  259. Glickman, M.S., Cox, J.S. and Jacobs, W.R., Jr. (2000) A Novel Mycolic Acid Cyclopropane Synthetase Is Required for Cording, Persistence and Virulence of Mycobacterium tuberculosisMolec. Cell. 5:717-727. PMCID: PMC in process.

  260. Mediavilla, J., Jain, S., Kriakov, J., Ford, M.E., Jacobs, W.R., Jr., Hendrix, R. W. and Hatful, G.F. (2000) Genome Organization and Characterization of Mycobacteriophage Bxb1. Mol. Microbiol. 38: 955-970. PMCID: PMC in process.

  261. Chambers, M.A., Williams, A., Gavier-Widen, D., Whelan, A., Hall, G., Marsh, P.D., Bloom, B.R., Jacobs, W.R., Jr. and Hewinson, R.G. (2000) Identification of a BCG Auxotrophic Mutant Which Protects Guinea Pigs Against Tuberculosis Without Sensitising to Tuberculin.  Infect. Immun. 68:7094-7099. PMCID: PMC97820.

  262. Zimhony, O., Cox, J.S., Welch, J.T., Vilchèze, C. and Jacobs, W.R., Jr.  (2000)  Pyrazinamide Inhibits the Eukaryotic-Like Fatty Acid Synthetase I (FASI) of Mycobacterium tuberculosis. Nat. Med. 6:1043-1047. PMCID: PMC in process.

  263. Sharma, V., Sharma, S., Hoener zu Bentrup, K., McKinney, J. D., Russell, D.G., Jacobs, W. R., Jr., and Sacchettini, J.C. (2000) The Structure of Isocitrate Lyase from Mycobacterium tuberculosis. Nat. Struct. Biol. 7:663-668. PMCID: PMC in process.

  264. McKinney, J.D., Honer zu Bentrup, K., Munoz-Elias, E.J.,  Miczak, A., Chen, B., Chan, W.-T., Swenson, D., Sacchettini, J.C., Jacobs, W.R., Jr. and Russell, D.G.  (2000) Persistence of Mycobacterium tuberculosis in Macrophages and Mice Requires the Glyoxylate Shunt Enzyme Isocitrate Lyase. Nature 406:735-738. PMCID: PMC in process.

  265. Vilchèze, C., Morbidoni, H.R., Weisbrod, T.R., Iwamoto, H., Sachettini, J.C. and Jacobs, W.R., Jr. (2000) Inactivation of the inhA-Encoded Fatty Acid Synthase II (FASII) Enoyl-Acyl Carrier Protein Reductase Induces the Accumulation of the FASI End Products and Cell Lysis of Mycobacterium smegmatisJ. Bacteriol. 182:4059-4067. PMCID: PMC94593.

  266. Braunstein, M., Griffin, T.J., IV, Kriakov, J.I., Friedman, S.T., Grindley, N.D.F. and Jacobs W.R., Jr. (2000) Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552'phoA in vitro Transposition System.  J. Bacteriol. 182:2732-2740. PMCID: PMC101980.

  267. Kremer, L., Douglas, D., Baulard, A.R., Morehouse, C., Guy, M., Alland, D., Dover, L., Lakey, J., Jacobs, W.R., Jr.,  Brennan, P.J., Minnikin, D.E. and Besra, G.S. (2000) Thiolactomycin and Related-structures as Novel Antimycobacterial Drugs That Target KasA and KasB in Mycobacterium tuberculosisJ. Biol. Chem. 275:16857-16864. PMCID: PMC in process.

  268. Hondalus, M.K., Bardarov, S.S., Russell, R., Chan, J., Jacobs, W.R., Jr. and Bloom, B.  (2000) Attenuation Of And Protection Induced By A Leucine Auxotroph of Mycobacterium tuberculosis. Infect. Immun. 68:2888-2898. PMCID: PMC97501.

  269. Alland, D., Steyn, A.J., Weisbrod, T., Aldrich, K., and Jacobs, W.R., Jr.  (2000) Characterization of a Mycobacterium tuberculosis iniBAC Promoter, a Promoter That Responds to Cell Wall Biosynthesis Inhibition.  J. Bacteriol. 182:1802-1811. PMCID: PMC101861.

  270. Piatek, A.S., Telenti, A., Murray, R.R., El-Haji, H., Jacobs, W.R., Jr., Kramer, F.R. and Alland, D. (2000) Genotypic Analysis of Mycobacterium tuberculosis in Two Distinct Populations Using Molecular Beacons:  Implications for Rapid Susceptibility Testing. Antimicrob. Agents Chemother. 44:103-110. PMCID: PMC89635.

  271. Cox, J.S., Chen, B., McNeil, M. and Jacobs, W.R., Jr.  (1999) Complex Lipid Determines Tissue-Specific Replication of Mycobacterium tuberculosis in Mice. Nature 402:79-83. PMCID: PMC in process.

  272. Pavelka, M.S., and Jacobs, W.R., Jr.  (1999) A Comparison of the Construction of Unmarked Deletion Mutations in Mycobacterium smegmatis, M. bovis Bacille Calmette-Guerin (BCG), and M. tuberculosis H37R by Allelic Exchange. J. Bacteriol. 181:4780-4789. PMCID: PMC93962.

  273. Bange, F.-C., Collins, F.M. and Jacobs, W.R., Jr.  (1999) Survival of Mice Infected with Mycobacterium smegmatis Containing Large DNA Fragments from Mycobacterium tuberculosisTuber. Lung Dis. 79:171-180. PMCID: PMC in process.

  274. Daugelat, S. and Jacobs, W.R. Jr.  (1999)The Mycobacterium tuberculosis recA intein Can be Used in  an  ORFTRAP to Select for Open Reading Frames.  Protein Sci8:644-653. PMCID: PMC2144272.

  275. Dussurget, O., Timm, J., Gomez, M., Gold, B., Yu, S., Sabol, S.Z., Holmes, R.K., Jacobs, W.R., Jr. and Smith, I.  (1999) Transcriptional Control of the Iron-Responsive fxbA Gene by the Mycobacterial Regulator IdeR. J. Bacteriol. 181:3402-3408. PMCID: PMC93806.

  276. Riska, P.F., Yu, S., Bardarov, S., Freundlich, L., Sarkis, G., Hatfull, G., Carriere, C., Kumar, V., Chan, J. and Jacobs, W.R., Jr.  (1999) Rapid Film-Based Determination of Antibiotic Susceptibilities of Mycobacterium tuberculosis Strains by Using a Luciferase Reporter Phage and the Bronx Box.  J. Clin. Microbiol37:1144-1149. PMCID: PMC88662.

  277. Yu, S., Fiss, E. and Jacobs, W.R., Jr.  (1998) Analysis of the Exochelin Locus in Mycobacterium smegmatis: Biosynthesis Genes Have Homology with Genes of the Peptide Synthetase Family.  J. Bacteriol. 180:4676-4685. PMCID: PMC107483.

  278.   Alland, D., Kramnik, I., Weisbrod, T.R., Otsubo, L., Cerny, R., Miller, L.P., Jacobs, W.R., Jr., and Bloom, B.R.  (1998) Identification of Differentially Expressed mRNA in Prokaryotic Organisms by Customized Amplification Libraries (DECAL): The Effect of Isoniazid on Gene Expression in Mycobacterium tuberculosisProc. Natl. Acad. Sci.USA95:13227-13232. PMCID: PMC23765.

  279. Basso, L.A., Zheng, R., Musser, J., Jacobs, W.R., Jr. and Blanchard, J.S.  (1998) Mechanisms of Isoniazid Resistance in M. tuberculosis: Enzymatic Characterization of Enoyl Reductase Mutants Identified in Isoniazid-Resistant Clinical Isolates.  J. Infect. Dis. 178:769-775. PMCID: PMC in process.

  280. Banerjee, A.,  Sugantino, M., Sacchettini, J.C. and Jacobs, W.R., Jr. (1998) The mabA Gene from the InhA Operon of M. tuberculosis Encodes a 3-keotacyl Reductase that Fails to Confer Isoniazid Resistance.  Microbiology  144:2697-2707. PMCID: PMC in process.

  281. Miesel, L., Weisbrod, T.R., Marcinkeviciene, J.,  Bittman, R. and W.R. Jacobs, Jr.  (1998) NADH Dehydrogenase Defects Confer Isoniazid Resistance and Conditional Lethality in Mycobacterium smegmatisJ. Bacteriol180:2459-2467. PMCID: PMC107089.

  282. Klabunde, T., Sharma, S., Telenti, A., Jacobs, W.R., Jr. and Sacchettini, J.C.  (1998)  Crystal Structure of gyrA intein from Mycobacterium xenopi Reveals Structural Basis of Protein Splicing.  Nat. Struct. Biol5:31-36. PMCID: PMC in process.

  283. Rozwarski, D., Grant, G., Barton, D., Jacobs, W.R., Jr.  and Sacchettini, J.C.  (1998) Modification of the NADH of the Isoniazid Target (InhA) from Mycobacterium tuberculosisScience  279:98-102. PMCID: PMC in process.

  284. Riska, P.F., Jacobs, W.R., Jr.,  Bloom, B.R., McKitrick J. and Chan, J.  (1997) Specific Identification of Mycobacterium tuberculosis with the Luciferase Reporter Mycobacteriophage:  Use of p-Nitro-Acetylamino-Hydroxy Propiophenone.  J. Clin. Microbiol35:3225-3231. PMCID: PMC230152

  285. Carriere, C., Kriakov, J., Riska, P., Chan, J., Bardarov, S. and Jacobs, W.R., Jr.  (1997) Conditionally-Replicating Luciferase Reporter Phages:  Improved Sensitivity for Rapid Detection and Assessment of Drug Susceptibility of Mycobacterium tuberculosisJ. Clin. Microbiol35:3232-3239. PMCID: PMC230153.

  286. Telenti, A., Southworth, M., Alcaide, F., Daugelat, S.,  Jacobs, W.R., Jr. and Perler, F.B.  (1997) The Mycobacterium xenopi GyrA Protein Splicing Element: Characterization of a Minimal Intein.  J. Bacteriol179:6378-6382. PMCID: PMC179553.

  287. Pelicic, V.,  Jackson, M., Reyrat, J.M.,  Jacobs, W.R., Jr., Gicquel, B.  and Guilhot, C.  (1997)  Efficient Allelic Exchange and Transposon Mutagenesis in Mycobacterium tuberculosisProc. Natl. Acad. Sci., USA. 94:10955-10960. PMCID: PMC23543.

  288. Bardarov, S.,  Kriakov, J., Carriere, C.,  Yu, S., Vaamonde, C.,  McAdam, R.A.,  Bloom, B.R.,  Hatfull, G.F.  and Jacobs, W.R., Jr.  (1997)  Conditionally Replicating Mycobacteriophages: A System for Transposon Delivery to Mycobacterium tuberculosisProc. Natl. Acad. Sci., USA. 94:10961-10966. PMCID: PMC23545.

  289. Sreevatsan, S., Stockbauer, K.E.,  Pan, X.,  Kreiswirth, B.,  Moghazeh, S.L.,  Jacobs, W.R., Jr., Telenti, A. and J.M. Musser  (1997)  Ethambutol Resistance in Mycobacterium tuberculosis Critical Role of embB Mutations.  Antimicrob. Agents Chemother. 41:1677-1681.PMCID: PMC163984.

  290. Telenti, A., Philipp, W., Seevatsan S., Bernasconi C., Stockbauer, K., Wieles, B., Musser, J.  and Jacobs, W.R., Jr.  (1997)  The emb Operon, a Gene Cluster of Mycobacterium tuberculosis Involved in Resistance to Ethambutol.  Nat. Med.  3:567-570. PMCID: PMC179553.

  291. Cirillo, J.D., Weisbrod, T.R., Banerjee, A., Bloom, B.R. and Jacobs, W.R., Jr. (1997) Genetic determination of meso-diaminapimelate biosynthetic pathway of mycobacteria.  J. Bacteriol. 179:2792. PMCID: PMC179036.

  292. Tuckman, D.,  Donnelly, R.J.,  Jacobs, W.R., Jr. and Connell, N.D.  (1997) Interruption of the Phosphoglucose Isomerase Gene Results in Glucose Auxotrophy  in Mycobacterium smegmatis. J. Bacteriol179:2724-2730. PMCID: PMC179023.

  293. Pavelka, M.S., Weisbrod, T. and Jacobs, W.R., Jr. (1997) Cloning of the dapB Gene Encoding Dihydrodipicolinate Reductase, from Mycobacterium tuberculosis. J. Bacteriol179:2777-2782. PMCID: PMC179033.

  294. Pavelka, M.S., Jr. and Jacobs, W.R., Jr. (1996) Biosynthesis of Diaminopimelate (DAP), the Precursor of Lysine and a Component of Peptidoglycan, Is an Essential Function of Mycobacterium smegmatisJ. Bacteriol. 178:6496-6507. PMCID: PMC178536.

  295. Dubnau, E., Soares, S.,  Huang, T.J. and Jacobs, W.R., Jr. (1996) Overproduction of Mycobacterial Ribosomal Protein S13 Induces Catalase/Peroxidase Activity and Hypersensitivity to Isoniazid in Mycobacterium smegmatis. Gene 170:17-22. PMCID: PMC in process.

  296. Bange, F-C.,  Brown, A.M. and Jacobs, W.R., Jr.  (1996) Leucine Auxotrophy Restricts Growth of Mycobacterium bovis BCG in Macrophages.  Infect. Immun. 64:1794-1799.PMCID: PMC173994.

  297. Hewinson, R.G.,  Michell, S.L.,  Russell, W.P.  McAdam, R.A. and Jacobs, W.R., Jr.  (1996) Molecular Characterization of MPT83: A Seroreactive Antigen of Mycobacterium tuberculosis with Homology to MPT70. Scand. J. Immunol43:490-499. PMCID: PMC in process.

  298. Pearson, R.E., Jurgensen, S.,  Sarkis, G.J.,  Hatfull, G.F. and Jacobs, W.R., Jr.  (1996) Construction of D29 Shuttle Plasmids and Luciferase Reporter Phages for Detection of Mycobacteria. Gene 183:129-136. PMCID: PMC in process.

  299. Phillip, W.J.,  Poulet, S., Eiglmeier, K.,  Pascopella, L., Balasubramanian, V., Heym, B., Bergh, S.,  Bloom, B.R.  Jacobs, W.R., Jr.  and Cole, S.T.  (1996) An Integrated Map of the Genome of the Tubercle Bacillus Mycobacterium tuberculosis H37Rv, and Comparison with M. leprae. Proc. Natl. Acad. Sci. USA93:3132-3137. PMCID: PMC39774.

  300. Guleria, I., Teitelbaum, R., McAdam, R.A., Kalpana, G., Jacobs, W.R., Jr. and Bloom, B.R. (1996) Auxotrophic Vaccines for Tuberculosis.   Nat. Med. 2:334-337. PMCID: PMC in process.

  301. Balasubramanian, V.,  Pavelka, Jr., M.S., Bardarov, S., Martin, J.,  Weisbrod, T.R.,  McAdam, R.A.,  Bloom, B.R. and Jacobs, W.R., Jr. (1996) Allelic Exchange in Mycobacterium tuberculosis with Long Linear Recombination  Substrates. J. Bacteriol. 178:273-279. PMCID: PMC177649.

  302. Takiff, H., Ciminio, M., Delgado, M., Musso, M.C., Salazar, L., Martinez, R.,  Telenti, A., Bloom, B.R. and Jacobs, W.R., Jr. (1996) Efflux Pump of the Proton Antiporter Family Confers Low-level Fluoroquinoline Resistance in Mycobacterium smegmatis. Proc. Natl. Acad. Sci.USA. 93:362-366. PMCID: PMC40238.

  303. Quemard, A., Dessen, A.,  Sugantino, M.,  Jacobs, W.R., Jr.,  Sacchettini, J.C. and Blanchard, J.S. (1996) Binding of Catalase Peroxidase-Activated Isoniazid to Wild-Type and Mutant Mycobacterium tuberculosis Enoyl-ACP-Reductases.  J. Am. Chem. Soc. 118: 1561-1562. PMCID: PMC n/a.

  304. Collins, D.M., Kawakami, R.P., de Lisle, G.W., Pascopella, L.,  Bloom, B.R. and Jacobs, W.R., Jr. (1995) Mutation  in the Principal Sigma Factor Causes a Loss of Virulence in a Strain of the Mycobacterium tuberculosis Complex   Proc. Natl. Acad. Sci. USA.  92:8036-8040.PMCID: PMC41281.

  305. Quemard, A.,  Sacchettini, J.C.,  Dessen, A., Vilcheze, C., Bittman, R., Jacobs, W.R., Jr. and Blanchard, J.S. (1995) Enzymatic Characterization of the Target for Isoniazid in Mycobacterium tuberculosis. Biochemistry. 34:8235-8241. PMCID: PMC in process.

  306. Dessen, A., Quemard, A., Blanchard, J.S., Jacobs, W.R., Jr. and Sacchettini, J.C. (1995) Crystal Structure and Function of the Isoniazid Target of Mycobacterium tuberculosis. Science267:1638-1641. PMCID: PMC in process.

  307. McAdam, R.A., Weisbrod, T.R., Martin, J., Scuderi, J.D., Brown, A, Cirillo, J.D., Kalpana, G.,  Bloom, B.R. and Jacobs, W.R., Jr. (1995) In vivo Growth Characteristics of Leucine and Methionine Auxotrophic Mutants of Mycobacterium bovis BCG Generated by Transposon Mutagenesis. Infect. Immun. 63: 1004-1012. PMCID: PMC173102.

  308. Kapur, V., Li, L.L.,  Hamrick, M.R., Pikaytis, B.B., Shinnick, T.M., Telenti, A., Jacobs, W.R., Jr.,  Banerjee, A., .Cole, S.T., Yuen, K.Y., Claridge, J.E., III, Kreiswirth, B.N. and Musser, J.M.  (1995) Rapid Mycobacterium Species Assignment and Unambiguous Identification of Mutations Associated with Antimicrobial Resistance in Mycobacterium tuberculosis by Automated DNA Sequencing.  Arch. Pathol. Lab. Med. 119:131-138. PMCID: PMC in process.

  309. Cirillo, J.D., Stover, C.K., Bloom, B.R., Jacobs, W.R. Jr., Barletta, R.G. (1995) Bacterial Vaccine Vectors and Bacillus Clmette-Guerin. Clin. Infect Dis  20:1001-1009. PMCID: PMC in process.

  310. Sarkis, G., Jacobs, W.R., Jr. and Hatfull, G.F. (1995) L5 Luciferase Reporter Mycobacteriophages: a Sensitive Tool for the Detection and Assay of Live Mycobacteria. Mol. Microbiol. 15:1055-1067. PMCID: PMC in process.

  311. Banerjee, A., Dubnau, E., Quemard, A., Balasubramanian, V., Um, K.S., Wilson, T., Collins, D., de Lisle, G. and Jacobs, W.R., Jr.  (1994) InhA, a Gene Encoding a Target for Isoniazid and Ethionamide in Mycobacterium tuberculosis. Science 263: 227-230. PMCID: PMC in process.

  312. Cirillo, J.D., Weisbrod, T.R., Pascopella, L., and Jacobs, W.R., Jr. (1994) Isolation and Characterization of the Aspartate Semialdehyde Dehydrogenase and Aspartokinase Genes From Mycobacteria. Molec. Microbiol. 11:629-639. PMCID: PMC in process.

  313. Pascopella, L., Collins, F.M., Martin, J.M., Stover, C.K., Lee, M.H., Hatfull, G.F., Bloom, B.R. and Jacobs, W.R., Jr.  (1994) Use of In vivo Complementation in Mycobacterium tuberculosis to Identify a Genomic Fragment Associated with Virulence. Infect. Immun. 62: 1313-1319. PMCID: PMC186277.

  314. Fiss, E.H., Yu, S., and Jacobs, W.R., Jr. (1994) Identification of Genes Involved in the Sequestration of Iron in Mycobacteria: the Ferric Exochelin Biosynthesis and Uptake Pathways. Mol. Microbiol. 14:557-569. PMCID: PMC in process.

  315. Mills, J.A., McNeil, M.R., Jacobs, W.R., Jr., and Brennan, P.J.  (1994) Genetic Loci Mycobacterium avium ser2 Gene Cluster and their Functions. J. Bacteriol. 176: 4803-4808. PMCID: PMC196313.

  316. Heym. B., Honore, N., Truffot-Pernot, C., Banerjee, A,M Jacobs, W.R., Jr., van Embden, J.D.A.,  Grosset, J.H. and Cole, S.T. (1994) Implications of Multidrug Resistance for the Future of Short-course Chemotherapy of Tuberculosis: a Molecular Study. Lancet. 344: 293-298. PMCID: PMC in process.

  317. Cirillo, J.D., Weisbrod, T.R., Banerjee, A.,  Bloom, B.R. and Jacobs, W.R., Jr. (1994) Genetic Determination of the meso-Diaminopimelate Biosynthetic Pathway of Mycobacteria. J. Bacteriol. 176: 4424-4429. PMCID: PMC205656.

  318. Brunhuber, N.W.W., Banerjee, A., Jacobs, W.R., Jr. and J.S. Blanchard.  (1994) Cloning, Sequencing, and Expression of Rhodococcus L-Phenylalanine Dehydrogenase: Sequence Comparisons to Amino Acid Dehydrogenases. J. Biol. Chem. 269:16203-16211. PMCID: PMC in process.

  319. Takiff, H.E., Salazar, L., Guerrero C., Huang, W.M., Kreisworth, B.,  Cole, S.T., Jacobs, W.R., Jr., and Telenti, A. (1994) Cloning and Nucleotide Sequence of Mycobacterium tuberculosis gyrA and gyrB Genes and Detection of Quinolone Resistance Mutations. Antimicrob. Agents Chemo. 38: 773-780. PMCID: PMC284541.

  320. Donnelly-Wu, M.K., Jacobs, W.R., Jr. and Hatfull, G.F. (1993) Superinfection Immunity of Mycobacteriophage L5: Applications for Genetic Transformation of Mycobacteria. Mol. Microbiol. 7:407-417. PMCID: PMC in process.

  321. Cirillo, J.D., Weisbrod, T.R. and Jacobs, W.R., Jr. (1993) Efficient Electro-Transformation of Mycobacterium smegmatis. Biorad Tech. Bull. 1360. PMCID: PMC in process.

  322. Belisle, J.T., Klaczkiewicz, K., Brennan, P.J., Jacobs, W.R., Jr., and Inamine, J. (1993) Rough Morphological Variants of Mycobacterium avium: Characterization of Genetic Deletions Resulting in the Loss of Glycopeptidolipid Expression.  J. Biol. Chem268:10517-10523.PMCID: PMC in process.

  323. Jacobs, W.R., Jr., Barletta, R., Udani, R., Chan, J.,  Kalkut, G., Sarkis, G., Hatfull, G.F. and Bloom, B.R. (1993) Rapid Assessment of Drug Susceptibilities of Mycobacterium tuberculosis by Means of Luciferase Reporter Phages. Science 260:819-822. PMCID: PMC in process.

  324. Cooksey, R.C., Crawford, J.T., Jacobs, W.R., Jr., and Shinnick, T.M. (1993) A Rapid Method for Screening Antimicrobial Agents for Activities Against a Strain of Mycobacterium tuberculosis Expressing Firefly Luciferase. Antimicrob. Agents Chemo. 37: 1348-1352. PMCID: PMC187964.

  325. Averill, L.E., Cavallo, U., Wallis, R.S., Boom, W.H.,  Bona, M., Mincek, M., Pascopella, L., Jacobs, W.R., Jr., and Ellner, J.J.  (1993) Screening of a Cosmid Library of Mycobacterium bovis BCG in smegmatis for Novel T-cell Stimulatory Antigens.  Res. Microbiol.  144:349-362. PMCID: PMC in process.

  326. Pascopella, L., Collins, F.M., Martin, J.M.,  Jacobs, W.R., Jr. and Bloom, B.R.  (1993) Identification of a Genomic Fragment of Mycobacterium tuberculosis Responsible for in vivo Growth Advantage. Infect. Agents Dis. 4:282-284. PMCID: PMC in process.

  327. 329.      Barletta, R.G., Kim, D.D., Snapper, S.B., Bloom, B.R. and Jacobs, W.R., Jr. (1992) Identification of Expression Signals of the Mycobacteriophages Bxb1, L1, and TM4 Using the Escherichia-Mycobacterium Shuttle Plasmids pYUB75 and pYUB76 Designed to Create Translational Fusions to the lacZ Gene. J. Gen. Microbiol. 138:23-30. PMCID: PMC in process.

  328. Daley, C.D., Small, P.M., Schecter, G.F., Schoolnick, G.K., McAdam, R.A., Jacobs, W.R., Jr.  and Hopewell, P.C.  (1992) An Outbreak of Tuberculosis with Accelerated Progression among Persons Infected with the Human Immunodeficiency Virus: an Analysis Using Restriction Fragment Length Polymorphisms. N. Engl. J. Med. 326:231-235. PMCID: PMC in process.

  329. Kalpana, V.G., Bloom, B.R. and Jacobs, W.R., Jr. (1991)  Insertional Mutagenesis and Illegitimate Recombination in Mycobacteria. Proc. Natl. Acad. Sci., USA.  88:5433-5437.PMCID: PMC51887.

  330.  Lee, M.H., Pascopella, L., Jacobs, W.R., Jr. and Hatfull, G.F. (1991) Site‑specific Integration of Mycobacteriophage L5: Integration‑Proficient Vectors for Mycobacterium smegmatis, BCG, and M. tuberculosis. Proc. Natl. Acad. Sci., USA. 88:3111-3115. PMCID: PMC51395.

  331. Sirawaraporn, W.,  Sirawaraporn, A., Chanpongsn, A., Jacobs, W.R.,  Jr. and Santi, D.V.  (1991)  Purification and Characterization of Dihydrofolate Reductase from Wild‑type and Trimethoprim‑Resistant Mycobacterium smegmatis. Exp. Parasitol. 72:184-190.PMCID: PMC in process.

  332. Stover, C.K.,  de la Cruz, V.F.,  Fuerst, T.R.,  Burlein, J.E.,  Benson, L.A.,  Bennett, L.T.,  Bansal, G.P.,  Young, J.F.,  Lee, M.H.,  Hatfull, G.,  Snapper, S.B., Barletta, R.G., Jacobs, W.R., Jr. and Bloom, B.R. (1991)  New Use of BCG for Recombinant Vaccines. Nature 351:456-460.PMCID: PMC n/a.

  333. Belisle, J.T., Pascopella, L., Imamine, J.,  Brennan, P.J. and  Jacobs, W.R., Jr. (1991) Isolation and Expression of a Gene cluster Responsible for Biosynthesis of the Glycopeptidolipid Antigen of Mycobacterium avium. J. Bacteriol. 173:6991-6997.PMCID: PMC209054.

  334. Cirillo, J.D., Barletta, R.G., Bloom, B.R. and W. R. Jacobs, Jr. (1991) A Novel Transposon Trap for Mycobacteria: Isolation and Characterization of IS1096. J. Bacteriol. 173:7772-7780.PMCID: PMC212567.

  335. Snapper, S.B., Melton, R.E., Mustafa, S., Kieser, T. and Jacobs, W.R., Jr. (1990) Isolation and Characterization of Efficient Plasmid Transformation Mutants of Mycobacterium smegmatis. Mol. Microbiol. 4:1911‑1919. PMCID: PMC in process.

  336. Barletta, R.G., Snapper, S.B.,  Cirillo, J.D.,  Connell, N.D.,  Kim, D.D., Jacobs,        W.R., Jr. and Bloom, B.R. (1990)  Recombinant BCG as a Candidate Oral Vaccine Vector. Res. Microbiol. 141:931‑939.PMCID: PMC in process.

  337. Lugosi, L., Jacobs, W.R., Jr., and Bloom, B.R. (1989) Genetic Transformation of BCG. Tubercle 70:159‑170. PMCID: PMC in process.

  338. Grosskinsky, C.M., Jacobs, W.R., Jr., Clark‑Curtiss, J.E., and Bloom, B.R. (1989) Genetic relationships Between Mycobacterium leprae, Mycobacterium tuberculosis, and Candidate Leprosy Vaccine Strains by DNA Hybridization:  Identification of a M. leprae‑Specific Repetitive Sequence. Infect. Immun. 57:1535‑1541. PMCID: PMC313310.

  339. Snapper, S.B., Lugosi, L.,  Jekkel, A.,  Melton, R.,  Kieser, T.,  Bloom, B.R.  and Jacobs, W.R.,  Jr. (1988)  Lysogeny and Transformation of Mycobacteria: Stable Expression of Foreign Genes. Proc. Natl. Acad. Sci., USA. 85:6987‑6991. PMCID: PMC282104.

  340. Jacobs, W.R., Jr., Tuckman, M. and Bloom, B.R. (1987) Introduction of Foreign DNA into Mycobacteria Using a Shuttle Phasmid. Nature 327:532‑536. PMCID: PMC in process.

  341. Jacobs, W.R., Jr., Docherty, M.A., Curtiss, R. III, and Clark‑Curtiss, J.E. (1986)  Expression of Mycobacterium leprae Genes from a Streptococcus mutans Promoter in Escherichia coli K‑12. Proc. Natl. Acad. Sci. USA. 83:1926‑1930. PMCID: PMC323197.

  342. Jacobs, W.R., Jr., Barrett, J.F., Clark‑Curtiss, J.E. and Curtiss R.  III. (1986) In vivo Repackaging of Recombinant Cosmid Molecules for Analysis of Salmonella typhimurium, Streptococcus mutans, and Mycobacterial Genomic Libraries. Infect. Immun. 52:101‑109.PMCID: PMC262204.

  343. Clark‑Curtiss, J.E., Jacobs, W.R., Jr., Docherty, M.A., Ritchie, L.R. and Curtiss, R. III. (1985)  Molecular Analysis of DNA and Construction of Genomic Libraries of Mycobacterium leprae. J. Bacteriol. 161:1093‑1102. PMCID: PMC215012